Bone mineral and collagen quality in humeri of ovariectomized cynomolgus monkeys given rhPTH(1-34) for 18 months

Eleftherios P. Paschalis, David Burr, Richard Mendelsohn, Janet M. Hock, Adele L. Boskey

Research output: Contribution to journalArticle

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Abstract

A recent study of ovariectomized monkeys, treated with recombinant human parathyroid hormone (rhPTH)(1-34) at 1 or 5 mg/kg/day for 18 months or for 12 months followed by 6 months withdrawal from treatment, showed significant differences in the geometry and histomorphometry of cortical bone of the midshaft humerus. To determine the extent to which the rapid bone turnover and cortical porosity induced by rhPTH(1-34) in ovariectomized monkeys modified mineral content, mineral crystal maturity and collagen maturity (cross-link distribution) in the cortical periosteal and endosteal regions, cross-sections of the cortical bone of the mid-humerus, were examined using Fourier transform infrared imaging (FTIRI). FTIRI analyses demonstrated that rhPTH(1-34) altered bone mineral and collagen properties in a dose-dependent manner. Mineral crystal maturity and collagen cross-link ratio (pyridinoline/dehydro-dihydroxylysinonorleucine) on both endosteal and periosteal surfaces decreased relative to ovariectomized animals, consistent with new bone formation. These changes were partially sustained after withdrawal of the higher dose of rhPTH(1-34), suggesting a prolonged after-effect on bone properties for at least two bone remodeling cycles. In conclusion, treatment of ovariectomized monkeys with rhPTH(1-34) had significant effects on cortical bone mineral-to-matrix ratio, mineral crystal maturity, and collagen cross-link ratio. These were fully reversible when the 1-μg rhPTH(1-34) treatment was withdrawn, but only partially reversed when the 5-μg rhPTH(1-34) dose was withdrawn.

Original languageEnglish
Pages (from-to)769-775
Number of pages7
JournalJournal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research
Volume18
Issue number4
StatePublished - Apr 1 2003

Fingerprint

Teriparatide
Macaca fascicularis
Humerus
Minerals
Collagen
Bone and Bones
Haplorhini
Bone Remodeling
Fourier Analysis
Porosity
Osteogenesis

Keywords

  • Bone quality
  • Infrared imaging
  • Osteoporosis
  • Ovariectomized monkey
  • Recombinant human parathyroid hormone (1-34)

ASJC Scopus subject areas

  • Surgery

Cite this

Bone mineral and collagen quality in humeri of ovariectomized cynomolgus monkeys given rhPTH(1-34) for 18 months. / Paschalis, Eleftherios P.; Burr, David; Mendelsohn, Richard; Hock, Janet M.; Boskey, Adele L.

In: Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, Vol. 18, No. 4, 01.04.2003, p. 769-775.

Research output: Contribution to journalArticle

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