Boundary segmentation for fluorescence microscopy using steerable filters

David Joon Ho, Paul Salama, Kenneth W. Dunn, Edward J. Delp

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Scopus citations

Abstract

Fluorescence microscopy is used to image multiple subcellular structures in living cells which are not readily observed using conventional optical microscopy. Moreover, two-photon microscopy is widely used to image structures deeper in tissue. Recent advancement in fluorescence microscopy has enabled the generation of large data sets of images at different depths, times, and spectral channels. Thus, automatic object segmentation is necessary since manual segmentation would be inefficient and biased. However, automatic segmentation is still a challenging problem as regions of interest may not have well defined boundaries as well as non-uniform pixel intensities. This paper describes a method for segmenting tubular structures in fluorescence microscopy images of rat kidney and liver samples using adaptive histogram equalization, foreground/background segmentation, steerable filters to capture directional tendencies, and connected-component analysis. The results from several data sets demonstrate that our method can segment tubular boundaries successfully. Moreover, our method has better performance when compared to other popular image segmentation methods when using ground truth data obtained via manual segmentation.

Original languageEnglish (US)
Title of host publicationMedical Imaging 2017
Subtitle of host publicationImage Processing
EditorsElsa D. Angelini, Martin A. Styner, Elsa D. Angelini
PublisherSPIE
ISBN (Electronic)9781510607118
DOIs
StatePublished - Jan 1 2017
EventMedical Imaging 2017: Image Processing - Orlando, United States
Duration: Feb 12 2017Feb 14 2017

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume10133
ISSN (Print)1605-7422

Other

OtherMedical Imaging 2017: Image Processing
CountryUnited States
CityOrlando
Period2/12/172/14/17

Keywords

  • Fluorescence microscopy
  • Image segmentation
  • Steerable filters
  • Tubule boundary

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

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  • Cite this

    Ho, D. J., Salama, P., Dunn, K. W., & Delp, E. J. (2017). Boundary segmentation for fluorescence microscopy using steerable filters. In E. D. Angelini, M. A. Styner, & E. D. Angelini (Eds.), Medical Imaging 2017: Image Processing [101330E] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 10133). SPIE. https://doi.org/10.1117/12.2254627