Bovine albumin release and degradation analysis of dicalcium phosphate dihydrate cement

Jeremy Metz, Peter Sargent, Tien Min G. Chu

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

Dicalcium phosphate dihydrate (DCPD) cement was effective in our prior study as a bone morphogenetic protein-2 (BMP-2) delivery vehicle in a rat segmental defect regeneration study. In this study, we investigated the effects of liquid-to-powder (L/P) ratio on the in vitro degradation and protein release behavior of this material. The L/P ratios used in this study ranged from 0.50 to 0.83. DCPD cylinders were formed with a diameter of 1/4" and a height of 1/4". The effect of L/P on the initial compressive strength was found to be related to the porosity of the material at different L/P level. The strength of the material in phosphate buffered solution was found to degrade roughly 20% in 14 days. The relation between the final porosity and the compressive strength after degradation was modeled with Ryshkewitch equation. A liquid-to-powder ratio of 0.55, 0.7, and 0.8 was then used to fabricate samples for the protein release kinetic study. The low porosity (L/P=0.55) group was found to have the fastest release rate, while the L/P=0.8 group had the lowest. More then 60% of the loaded protein was released after 10 hours in all three groups with a final total release ranging between 75% and 93%. The findings suggested that the protein release profile of DCPD cements can be adjusted by the L/P ratio.

Original languageEnglish (US)
Pages (from-to)296-301
Number of pages6
JournalBiomedical Sciences Instrumentation
Volume42
StatePublished - Apr 1 2006

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Keywords

  • Bovine albumin release profile
  • Compressive strength
  • Dicalcium phosphate dihydrate cement
  • Porosity
  • Tissue engineering

ASJC Scopus subject areas

  • Hardware and Architecture

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