Bradykinin modulates the electrophysiology of cultured rat sensory neurons through a pertussis toxin-insensitive G protein

Daniel S. Mcgehee, Gerry S. Oxford

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Application of the nonapeptide bradykinin (BK) to rat sensory neurons in culture elicits two types of electrical responses in a subpopulation of the cells: (i) an excitatory inward cation current (IBK), and (ii) an inhibition of voltage-activated calcium currents (ICa). Ion replacement experiments and current-voltage measurements indicate that IBK shows little selectivity for monovalent cations. We investigated the role of GTP-binding proteins in the BK signal transduction pathway. Internal dialysis of neurons with the inhibitory guanine nucleotide analogue, GDPβS, decreased the percentage of cells responding and the magnitude of the responses. This finding lead us to propose that a G protein is involved in this transduction pathway. We have attempted to identify the G protein subtype involved using pretreatment with the bacterial toxins pertussis toxin (PTX) and cholera toxin (CTX). PTX potentiated IBK slightly, but had no effect on the inhibition of ICa by BK. In contrast, PTX pretreatment blocked the effects of norepinephrine and neuropeptide Y on ICa, confirming that the PTX was capable of interfering with appropriate G protein signaling in these neurons. Pretreatment with CTX had no effect on the BK-induced inward current response. Therefore we propose that the BK signal transduction pathway utilizes a G protein that is not part of the Gi, Go, Gt, or Gs families.

Original languageEnglish (US)
Pages (from-to)21-30
Number of pages10
JournalMolecular and Cellular Neuroscience
Volume2
Issue number1
DOIs
StatePublished - 1991
Externally publishedYes

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Electrophysiology
Pertussis Toxin
Bradykinin
Sensory Receptor Cells
GTP-Binding Proteins
Cholera Toxin
Signal Transduction
Bacterial Toxins
Neurons
Monovalent Cations
Guanine Nucleotides
Neuropeptide Y
Cations
Dialysis
Norepinephrine
Ions
Calcium

ASJC Scopus subject areas

  • Molecular Biology
  • Cellular and Molecular Neuroscience
  • Developmental Neuroscience

Cite this

Bradykinin modulates the electrophysiology of cultured rat sensory neurons through a pertussis toxin-insensitive G protein. / Mcgehee, Daniel S.; Oxford, Gerry S.

In: Molecular and Cellular Neuroscience, Vol. 2, No. 1, 1991, p. 21-30.

Research output: Contribution to journalArticle

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