Brain homogenates from human tauopathies induce tau inclusions in mouse brain

Florence Clavaguera, Hiroyasu Akatsu, Graham Fraser, R. Anthony Crowther, Stephan Frank, Jürgen Hench, Alphonse Probst, David T. Winkler, Julia Reichwald, Matthias Staufenbiel, Bernardino Ghetti, Michel Goedert, Markus Tolnay

Research output: Contribution to journalArticle

286 Citations (Scopus)

Abstract

Filamentous inclusions made of hyperphosphorylated tau are characteristic of numerous human neurodegenerative diseases, including Alzheimer's disease, tangle-only dementia, Pick disease, argyrophilic grain disease (AGD), progressive supranuclear palsy, and corticobasal degeneration. In Alzheimer's disease and AGD, it has been shown that filamentous tau appears to spread in a stereotypic manner as the disease progresses. We previously demonstrated that the injection of brain extracts from human mutant P301S tau-expressing transgenic mice into the brains of mice transgenic for wild-type human tau (line ALZ17) resulted in the assembly of wild-type human tau into filaments and the spreading of tau inclusions from the injection sites to anatomically connected brain regions. Here we injected brain extracts from humans who had died with various tauopathies into the hippocampus and cerebral cortex of ALZ17 mice. Argyrophilic tau inclusions formed in all cases and following the injection of the corresponding brain extracts, we recapitulated the hallmark lesions of AGD, PSP and CBD. Similar inclusions also formed after intracerebral injection of brain homogenates from human tauopathies into nontransgenic mice. Moreover, the induced formation of tau aggregates could be propagated between mouse brains. These findings suggest that once tau aggregates have formed in discrete brain areas, they become self-propagating and spread in a prion-like manner.

Original languageEnglish
Pages (from-to)9535-9540
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume110
Issue number23
DOIs
StatePublished - Jun 4 2013

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Tauopathies
Brain
Injections
Transgenic Mice
Alzheimer Disease
Pick Disease of the Brain
Progressive Supranuclear Palsy
Prions
Neurodegenerative Diseases
Cerebral Cortex
Dementia
Hippocampus

ASJC Scopus subject areas

  • General

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Brain homogenates from human tauopathies induce tau inclusions in mouse brain. / Clavaguera, Florence; Akatsu, Hiroyasu; Fraser, Graham; Crowther, R. Anthony; Frank, Stephan; Hench, Jürgen; Probst, Alphonse; Winkler, David T.; Reichwald, Julia; Staufenbiel, Matthias; Ghetti, Bernardino; Goedert, Michel; Tolnay, Markus.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 110, No. 23, 04.06.2013, p. 9535-9540.

Research output: Contribution to journalArticle

Clavaguera, F, Akatsu, H, Fraser, G, Crowther, RA, Frank, S, Hench, J, Probst, A, Winkler, DT, Reichwald, J, Staufenbiel, M, Ghetti, B, Goedert, M & Tolnay, M 2013, 'Brain homogenates from human tauopathies induce tau inclusions in mouse brain', Proceedings of the National Academy of Sciences of the United States of America, vol. 110, no. 23, pp. 9535-9540. https://doi.org/10.1073/pnas.1301175110
Clavaguera, Florence ; Akatsu, Hiroyasu ; Fraser, Graham ; Crowther, R. Anthony ; Frank, Stephan ; Hench, Jürgen ; Probst, Alphonse ; Winkler, David T. ; Reichwald, Julia ; Staufenbiel, Matthias ; Ghetti, Bernardino ; Goedert, Michel ; Tolnay, Markus. / Brain homogenates from human tauopathies induce tau inclusions in mouse brain. In: Proceedings of the National Academy of Sciences of the United States of America. 2013 ; Vol. 110, No. 23. pp. 9535-9540.
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