Branched-chain α-keto acid dehydrogenase kinase content in rat skeletal muscle is decreased by endurance training

Hisao Fujii, Yoshiharu Shimomura, Taro Murakami, Naoya Nakai, Tasuku Sato, Masashige Suzuki, Robert Harris

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

The activity state of branched-chain α-keto acid dehydrogenase complex in skeletal muscle was elevated by running exercise in trained and untrained rats, but level of this elevation was significantly greater in the former than in the latter. To elucidate the mechanism of the training effect on the exercise-induced activation of the complex, a protein amount of branched chain α-keto acid dehydrogenase kinase, which is responsible for inactivation of the complex by phosphorylation, in the muscle was measured by the Western blot analysis. Endurance training decreased the content of the kinase protein in the muscle by ~30%, suggesting that this decrease is involved in the mechanisms for greater activation of the complex by exercise in trained rats.

Original languageEnglish
Pages (from-to)1211-1216
Number of pages6
JournalBiochemistry and Molecular Biology International
Volume44
Issue number6
StatePublished - 1998

Fingerprint

3-Methyl-2-Oxobutanoate Dehydrogenase (Lipoamide)
Muscle
Rats
Skeletal Muscle
Durability
Phosphotransferases
Muscle Proteins
Chemical activation
Running
Protein Kinases
Phosphorylation
Western Blotting
Muscles
Proteins

Keywords

  • Acute exercise
  • Branched-chain α-keto acid dehydrogenase complex
  • Branched-chain α-keto acid dehydrogenase kinase
  • Endurance training
  • Rat
  • Skeletal muscle

ASJC Scopus subject areas

  • Biochemistry
  • Genetics
  • Molecular Biology

Cite this

Branched-chain α-keto acid dehydrogenase kinase content in rat skeletal muscle is decreased by endurance training. / Fujii, Hisao; Shimomura, Yoshiharu; Murakami, Taro; Nakai, Naoya; Sato, Tasuku; Suzuki, Masashige; Harris, Robert.

In: Biochemistry and Molecular Biology International, Vol. 44, No. 6, 1998, p. 1211-1216.

Research output: Contribution to journalArticle

Fujii, Hisao ; Shimomura, Yoshiharu ; Murakami, Taro ; Nakai, Naoya ; Sato, Tasuku ; Suzuki, Masashige ; Harris, Robert. / Branched-chain α-keto acid dehydrogenase kinase content in rat skeletal muscle is decreased by endurance training. In: Biochemistry and Molecular Biology International. 1998 ; Vol. 44, No. 6. pp. 1211-1216.
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