Branched-chain α-ketoacid dehydrogenase kinase: Molecular cloning, expression, and sequence similarity with histidine protein kinases

Kirill M. Popov, Yu Zhao, Yoshiharu Shimomura, Martha J. Kuntz, Robert Harris

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Abstract

A cDNA for branched-chain α-ketoacid dehydrogenase kinase was cloned from a rat heart cDNA library. The cDNA had an open reading frame encoding a protein of 382 amino acid residues with a calculated molecular weight of 43,280. The clone codes for the branched-chain α-ketoacid dehydrogenase kinase based on the following: 1) the deduced amino acid sequence contained the partial sequence of the kinase determined by direct sequencing; 2) expression of the cDNA in Escherichia coli resulted in synthesis of a 43,000-Da protein that was recognized specifically by kinase antibodies; and 3) enzyme activity that phosphorylated and inactivated the branched-chain α-ketoacid dehydrogenase complex was found in extracts of E. coli expressing the protein. Northern blot analysis indicated the mRNA for the branched-chain α-ketoacid dehydrogenase kinase was more abundant in rat heart than in rat liver, as expected from the relative amounts of kinase activity expressed in these tissues. The deduced sequence of the kinase aligned with a high degree of similarity within subdomains characteristic of procaryotic histidine protein kinases. This first mitochondrial protein kinase to be cloned appears more closely related in sequence to procaryotic histidine protein kinases than to eucaryotic serine/threonine protein kinases.

Original languageEnglish
Pages (from-to)13127-13130
Number of pages4
JournalJournal of Biological Chemistry
Volume267
Issue number19
StatePublished - Jul 5 1992

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3-Methyl-2-Oxobutanoate Dehydrogenase (Lipoamide)
Cloning
Molecular Cloning
Histidine
Protein Kinases
Phosphotransferases
Rats
Complementary DNA
Amino Acids
Escherichia coli Proteins
Protein-Serine-Threonine Kinases
Mitochondrial Proteins
Enzyme activity
Histidine Kinase
Gene Library
Northern Blotting
Liver
Escherichia coli
Open Reading Frames
Amino Acid Sequence

ASJC Scopus subject areas

  • Biochemistry

Cite this

Branched-chain α-ketoacid dehydrogenase kinase : Molecular cloning, expression, and sequence similarity with histidine protein kinases. / Popov, Kirill M.; Zhao, Yu; Shimomura, Yoshiharu; Kuntz, Martha J.; Harris, Robert.

In: Journal of Biological Chemistry, Vol. 267, No. 19, 05.07.1992, p. 13127-13130.

Research output: Contribution to journalArticle

Popov, Kirill M. ; Zhao, Yu ; Shimomura, Yoshiharu ; Kuntz, Martha J. ; Harris, Robert. / Branched-chain α-ketoacid dehydrogenase kinase : Molecular cloning, expression, and sequence similarity with histidine protein kinases. In: Journal of Biological Chemistry. 1992 ; Vol. 267, No. 19. pp. 13127-13130.
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