Brg1 governs distinct pathways to direct multiple aspects of mammalian neural crest cell development

Wei Li, Yiqin Xiong, Ching Shang, Karen Y. Twu, Calvin T. Hang, Jin Yang, Pei Han, Chieh Yu Lin, Chien Jung Lin, Feng Chiao Tsai, Kryn Stankunas, Tobias Meyer, Daniel Bernstein, Minggui Pan, Ching-Pin Chang

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Development of the cerebral vessels, pharyngeal arch arteries (PAAs). and cardiac outflow tract (OFT) requires multipotent neural crest cells (NCCs) that migrate from the neural tube to target tissue destinations. Little is known about how mammalian NCC development is orchestrated by gene programming at the chromatin level, however. Here we show that Brahma-related gene 1 (Brg1), an ATPase subunit of the Brg1/Brahma-associated factor (BAF) chromatin-remodeling complex, is required in NCCs to direct cardiovascular development. Mouse embryos lacking Brg1 in NCCs display immature cerebral vessels, aberrant PAA patterning, and shortened OFT. Brg1 suppresses an apoptosis factor, Apoptosis signal-regulating kinase 1 (Ask1), and a cell cycle inhibitor, p21cip1, to inhibit apoptosis and promote proliferation of NCCs, thereby maintaining a multipotent cell reservoir at the neural crest. Brg1 also supports Myosin heavy chain 11 (Myh11) expression to allow NCCs to develop into mature vascular smooth muscle cells of cerebral vessels. Within NCCs, Brg1 partners with chromatin remodeler Chromodomain-helicase-DNA-binding protein 7 (Chd7) on the PlexinA2 promoter to activate PlexinA2, which encodes a receptor for semaphorin to guide NCCs into the OFT. Our findings reveal an important role for Brg1 and its downstream pathways in the survival, differentiation, and migration of the multipotent NCCs critical for mammalian cardiovascular development.

Original languageEnglish (US)
Pages (from-to)1738-1743
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume110
Issue number5
DOIs
StatePublished - Jan 29 2013
Externally publishedYes

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Neural Crest
Genes
Branchial Region
Chromatin
MAP Kinase Kinase Kinase 5
Arteries
Semaphorins
Apoptosis
Neural Tube
Chromatin Assembly and Disassembly
Myosin Heavy Chains
DNA-Binding Proteins
Vascular Smooth Muscle
Smooth Muscle Myocytes
Adenosine Triphosphatases
Cell Cycle
Embryonic Structures

ASJC Scopus subject areas

  • General

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Brg1 governs distinct pathways to direct multiple aspects of mammalian neural crest cell development. / Li, Wei; Xiong, Yiqin; Shang, Ching; Twu, Karen Y.; Hang, Calvin T.; Yang, Jin; Han, Pei; Lin, Chieh Yu; Lin, Chien Jung; Tsai, Feng Chiao; Stankunas, Kryn; Meyer, Tobias; Bernstein, Daniel; Pan, Minggui; Chang, Ching-Pin.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 110, No. 5, 29.01.2013, p. 1738-1743.

Research output: Contribution to journalArticle

Li, W, Xiong, Y, Shang, C, Twu, KY, Hang, CT, Yang, J, Han, P, Lin, CY, Lin, CJ, Tsai, FC, Stankunas, K, Meyer, T, Bernstein, D, Pan, M & Chang, C-P 2013, 'Brg1 governs distinct pathways to direct multiple aspects of mammalian neural crest cell development', Proceedings of the National Academy of Sciences of the United States of America, vol. 110, no. 5, pp. 1738-1743. https://doi.org/10.1073/pnas.1218072110
Li, Wei ; Xiong, Yiqin ; Shang, Ching ; Twu, Karen Y. ; Hang, Calvin T. ; Yang, Jin ; Han, Pei ; Lin, Chieh Yu ; Lin, Chien Jung ; Tsai, Feng Chiao ; Stankunas, Kryn ; Meyer, Tobias ; Bernstein, Daniel ; Pan, Minggui ; Chang, Ching-Pin. / Brg1 governs distinct pathways to direct multiple aspects of mammalian neural crest cell development. In: Proceedings of the National Academy of Sciences of the United States of America. 2013 ; Vol. 110, No. 5. pp. 1738-1743.
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