Bright red-emitting highly reliable styryl probe with large stokes shift for visualizing mitochondria in live cells under wash-free conditions

Chathura S. Abeywickrama, Kaveesha J. Wiesinghe, Robert V. Stahelin, Yi Pang

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

Bright red-emitting pyridinium cyanine based styryl probe 2 is synthesized in good yields. Probe 2 demonstrated a large Stokes’ shift (Δλ ≈ 128 nm, 4227 cm−1 in DCM) and excellent fluorescent quantum yield (Φf1 ≈ 0.2 - 0.7) due to strong Intra-molecular charge transfer (ICT). Probe 2 was found to exhibit exceptional selectivity for cellular mitochondria in both normal (COS-7) and cancer (A549) cell lines. Probe 2 is readily applicable as a “wash-free” dye to visualize mitochondria as it does not require post-staining washing prior to imaging. Styryl probe 2 also showed an excellent biocompatibility as the calculated LC50 (lethal concentration, 50%) value was > 20 μM. Probe 2 emission did not show any interferences from anionic species or other biological molecules. Probe 2 is readily excitable (λex∼460 and λem∼618 nm) with the available laser (454 nm) in commercial microscopes and thus it can be a useful probe for mitochondrial tracking in live cells.

Original languageEnglish (US)
Pages (from-to)76-83
Number of pages8
JournalSensors and Actuators, B: Chemical
Volume285
DOIs
StatePublished - Apr 15 2019
Externally publishedYes

Keywords

  • Biocompatibility
  • Intra-molecular charge transfer (ICT)
  • Large stokes shift
  • Mitochondria selectivity
  • Styryl dye
  • Wash-free application

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Instrumentation
  • Condensed Matter Physics
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Electrical and Electronic Engineering
  • Materials Chemistry

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