Brightness alters Heidelberg Retinal Flowmeter measurements in an in vitro model

Alexander C. Tsang, Alon Harris, Larry Kagemann, Hak Sung Chung, Bryan M. Snook, Hanna J. Garzozi

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

PURPOSE. The Heidelberg Retinal Flowmeter (HRF), a laser Doppler flowmetry device, has captured interest as a research and clinical tool for measurement of ocular blood flow. Concerns remain about the range and accuracy of the values that it reports. METHODS. An in vitro blood-flow model was constructed to provide well-controlled laminar flow through a glass capillary for assessment by HRF. A change in material behind the glass capillary was used to simulate changing brightness conditions between eyes. RESULTS. Velocities reported by the HRF correlated linearly to true velocities below 8.8 mm/sec. Beyond 8.8 mm/sec, HRF readings fluctuated randomly. True velocity and HRF reported velocities were highly correlated, with r = 0.967 (P < 0.001) from 0.0 mm/sec to 2.7 mm/sec mean velocity using a light background, and r = 0.900 (P < 0.001) from 2.7 mm/sec to 8.8 mm/sec using a darker background. However, a large change in the γ-intercept occurred in the calibration curve with the background change. CONCLUSIONS. The HRF may report velocities inaccurately because of varying brightness in the fundus. In the present experiment, a darker background produced an overreporting of velocities. An offset, possibly introduced by a noise correction routine, apparently contributed to the inaccuracies of the HRF measurements. Such offsets vary with local and global brightness. Therefore, HRF measurements may be error prone when comparing eyes. When used to track perfusion in a single eye over time, meaningful comparison may be possible if meticulous care is taken to align vessels and intensity controls to achieve a similar level of noise correction between measurements.

Original languageEnglish
Pages (from-to)795-799
Number of pages5
JournalInvestigative Ophthalmology and Visual Science
Volume40
Issue number3
StatePublished - 1999

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Flowmeters
Glass
Noise
Laser-Doppler Flowmetry
In Vitro Techniques
Calibration
Reading
Perfusion
Light
Equipment and Supplies

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Tsang, A. C., Harris, A., Kagemann, L., Chung, H. S., Snook, B. M., & Garzozi, H. J. (1999). Brightness alters Heidelberg Retinal Flowmeter measurements in an in vitro model. Investigative Ophthalmology and Visual Science, 40(3), 795-799.

Brightness alters Heidelberg Retinal Flowmeter measurements in an in vitro model. / Tsang, Alexander C.; Harris, Alon; Kagemann, Larry; Chung, Hak Sung; Snook, Bryan M.; Garzozi, Hanna J.

In: Investigative Ophthalmology and Visual Science, Vol. 40, No. 3, 1999, p. 795-799.

Research output: Contribution to journalArticle

Tsang, AC, Harris, A, Kagemann, L, Chung, HS, Snook, BM & Garzozi, HJ 1999, 'Brightness alters Heidelberg Retinal Flowmeter measurements in an in vitro model', Investigative Ophthalmology and Visual Science, vol. 40, no. 3, pp. 795-799.
Tsang, Alexander C. ; Harris, Alon ; Kagemann, Larry ; Chung, Hak Sung ; Snook, Bryan M. ; Garzozi, Hanna J. / Brightness alters Heidelberg Retinal Flowmeter measurements in an in vitro model. In: Investigative Ophthalmology and Visual Science. 1999 ; Vol. 40, No. 3. pp. 795-799.
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