Bronchoalveolar lavage fluid from copd patients reveals more compounds associated with disease than matched plasma

Eitan Halper-Stromberg, Lucas Gillenwater, Charmion Cruickshank-Quinn, Wanda Kay O’neal, Nichole Reisdorph, Irina Petrache, Yonghua Zhuang, Wassim W. Labaki, Jeffrey L. Curtis, James Wells, Stephen Rennard, Katherine A. Pratte, Prescott Woodruff, Kathleen A. Stringer, Katerina Kechris, Russell P. Bowler

Research output: Contribution to journalArticle

Abstract

Smoking causes chronic obstructive pulmonary disease (COPD). Though recent studies identified a COPD metabolomic signature in blood, no large studies examine the metabolome in bronchoalveolar lavage (BAL) fluid, a more direct representation of lung cell metabolism. We performed untargeted liquid chromatography–mass spectrometry (LC–MS) on BAL and matched plasma from 115 subjects from the SPIROMICS cohort. Regression was performed with COPD phenotypes as the outcome and metabolites as the predictor, adjusted for clinical covariates and false discovery rate. Weighted gene co-expression network analysis (WGCNA) grouped metabolites into modules which were then associated with phenotypes. K-means clustering grouped similar subjects. We detected 7939 and 10,561 compounds in BAL and paired plasma samples, respectively. FEV1 /FVC (Forced Expiratory Volume in One Second/Forced Vital Capacity) ratio, emphysema, FEV1 % predicted, and COPD exacerbations associated with 1230, 792, eight, and one BAL compounds, respectively. Only two plasma compounds associated with a COPD phenotype (emphysema). Three BAL co-expression modules associated with FEV1/FVC and emphysema. K-means BAL metabolomic signature clustering identified two groups, one with more airway obstruction (34% of subjects, median FEV1 /FVC 0.67), one with less (66% of subjects, median FEV1 /FVC 0.77; p < 2 × 10−4). Associations between metabolites and COPD phenotypes are more robustly represented in BAL compared to plasma.

Original languageEnglish (US)
Article number157
JournalMetabolites
Volume9
Issue number8
DOIs
StatePublished - Aug 2019

Fingerprint

Pulmonary diseases
Bronchoalveolar Lavage Fluid
Bronchoalveolar Lavage
Chronic Obstructive Pulmonary Disease
Plasmas
Fluids
Emphysema
Metabolites
Phenotype
Metabolomics
Cluster Analysis
Metabolome
Vital Capacity
Forced Expiratory Volume
Airway Obstruction
Electric network analysis
Metabolism
Spectrometry
Disease Progression
Spectrum Analysis

Keywords

  • BAL
  • BALF
  • Bronchoalveolar lavage
  • COPD
  • Emphysema
  • LC-MS
  • Mass spectrometry
  • Metabolomics
  • Plasma

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Biochemistry
  • Molecular Biology

Cite this

Halper-Stromberg, E., Gillenwater, L., Cruickshank-Quinn, C., O’neal, W. K., Reisdorph, N., Petrache, I., ... Bowler, R. P. (2019). Bronchoalveolar lavage fluid from copd patients reveals more compounds associated with disease than matched plasma. Metabolites, 9(8), [157]. https://doi.org/10.3390/metabo9080157

Bronchoalveolar lavage fluid from copd patients reveals more compounds associated with disease than matched plasma. / Halper-Stromberg, Eitan; Gillenwater, Lucas; Cruickshank-Quinn, Charmion; O’neal, Wanda Kay; Reisdorph, Nichole; Petrache, Irina; Zhuang, Yonghua; Labaki, Wassim W.; Curtis, Jeffrey L.; Wells, James; Rennard, Stephen; Pratte, Katherine A.; Woodruff, Prescott; Stringer, Kathleen A.; Kechris, Katerina; Bowler, Russell P.

In: Metabolites, Vol. 9, No. 8, 157, 08.2019.

Research output: Contribution to journalArticle

Halper-Stromberg, E, Gillenwater, L, Cruickshank-Quinn, C, O’neal, WK, Reisdorph, N, Petrache, I, Zhuang, Y, Labaki, WW, Curtis, JL, Wells, J, Rennard, S, Pratte, KA, Woodruff, P, Stringer, KA, Kechris, K & Bowler, RP 2019, 'Bronchoalveolar lavage fluid from copd patients reveals more compounds associated with disease than matched plasma', Metabolites, vol. 9, no. 8, 157. https://doi.org/10.3390/metabo9080157
Halper-Stromberg, Eitan ; Gillenwater, Lucas ; Cruickshank-Quinn, Charmion ; O’neal, Wanda Kay ; Reisdorph, Nichole ; Petrache, Irina ; Zhuang, Yonghua ; Labaki, Wassim W. ; Curtis, Jeffrey L. ; Wells, James ; Rennard, Stephen ; Pratte, Katherine A. ; Woodruff, Prescott ; Stringer, Kathleen A. ; Kechris, Katerina ; Bowler, Russell P. / Bronchoalveolar lavage fluid from copd patients reveals more compounds associated with disease than matched plasma. In: Metabolites. 2019 ; Vol. 9, No. 8.
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abstract = "Smoking causes chronic obstructive pulmonary disease (COPD). Though recent studies identified a COPD metabolomic signature in blood, no large studies examine the metabolome in bronchoalveolar lavage (BAL) fluid, a more direct representation of lung cell metabolism. We performed untargeted liquid chromatography–mass spectrometry (LC–MS) on BAL and matched plasma from 115 subjects from the SPIROMICS cohort. Regression was performed with COPD phenotypes as the outcome and metabolites as the predictor, adjusted for clinical covariates and false discovery rate. Weighted gene co-expression network analysis (WGCNA) grouped metabolites into modules which were then associated with phenotypes. K-means clustering grouped similar subjects. We detected 7939 and 10,561 compounds in BAL and paired plasma samples, respectively. FEV1 /FVC (Forced Expiratory Volume in One Second/Forced Vital Capacity) ratio, emphysema, FEV1 {\%} predicted, and COPD exacerbations associated with 1230, 792, eight, and one BAL compounds, respectively. Only two plasma compounds associated with a COPD phenotype (emphysema). Three BAL co-expression modules associated with FEV1/FVC and emphysema. K-means BAL metabolomic signature clustering identified two groups, one with more airway obstruction (34{\%} of subjects, median FEV1 /FVC 0.67), one with less (66{\%} of subjects, median FEV1 /FVC 0.77; p < 2 × 10−4). Associations between metabolites and COPD phenotypes are more robustly represented in BAL compared to plasma.",
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AU - Wells, James

AU - Rennard, Stephen

AU - Pratte, Katherine A.

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