C. elegans and H. sapiens mRNAs with edited 3′ UTRs are present on polysomes

Heather A. Hundley, Ammie A. Krauchuk, Brenda L. Bass

Research output: Contribution to journalArticlepeer-review

62 Scopus citations


Adenosine deaminases that act on RNA (ADARs) are editing enzymes that convert adenosine to inosine in double-stranded RNA (dsRNA). ADARs sometimes target codons so that a single mRNA yields multiple protein isoforms. However, ADARs most often target noncoding regions of mRNAs, such as untranslated regions (UTRs). To understand the function of extensive double-stranded 3′ UTR structures, and the inosines within them, we monitored the fate of reporter and endogenous mRNAs that include structured 3′ UTRs in wild-type Caenorhabditis elegans and in strains with mutations in the ADAR genes. In general, we saw little effect of editing on stability or translatability of mRNA, although in one case an ADR-1 dependent effect was observed. Importantly, whereas previous studies indicate that inosine-containing RNAs are retained in the nucleus, we show that both C. elegans and Homo sapiens mRNAs with edited, structured 3′ UTRs are present on translating ribosomes.

Original languageEnglish (US)
Pages (from-to)2050-2060
Number of pages11
Issue number10
StatePublished - Oct 2008


  • ADAR
  • Double-stranded RNA
  • Post-transcriptional regulation
  • RNA editing
  • Translation

ASJC Scopus subject areas

  • Molecular Biology

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