C-FLIP gene silencing eliminates tumor cells in breast cancer xenografts without affecting stromal cells

Travis W. Day, Anthony L. Sinn, Su Huang, Karen Pollok, George Sandusky, Ahmad Safa

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Cellular FLICE-like inhibitory protein (c-FLIP) is an inhibitor of death receptor-mediated apoptosis and exerts its anti-apoptotic function by blocking the activation of caspase-8. We recently showed that the siRNA-mediated knockdown of c-FLIP in MCF-7 breast cancer cells growing in vitro triggered apoptosis. The aim of this study was to determine if the in vivo knockdown of c-FLIP in MCF-7 breast cancer xenografts affected tumor viability. Immunohistochemical detection of c-FLIP in the tumor sections revealed that the knockdown of c-FLIP eliminated the neoplastic cells within the breast cancer xenografts without affecting the normal stromal and fibroblastic cells. These results indicate that c-FLIP is required for breast cancer growth and is a relevant therapeutic target for the treatment of breast cancer.

Original languageEnglish
Pages (from-to)3883-3886
Number of pages4
JournalAnticancer Research
Volume29
Issue number10
StatePublished - Oct 2009

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CASP8 and FADD-Like Apoptosis Regulating Protein
Gene Silencing
Stromal Cells
Heterografts
Breast Neoplasms
Neoplasms
Apoptosis
Death Domain Receptors
Caspase 8
Small Interfering RNA
Growth

Keywords

  • Apoptosis
  • c-FLIP
  • Cancer cell xenografts
  • Immunohistochemistry

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

C-FLIP gene silencing eliminates tumor cells in breast cancer xenografts without affecting stromal cells. / Day, Travis W.; Sinn, Anthony L.; Huang, Su; Pollok, Karen; Sandusky, George; Safa, Ahmad.

In: Anticancer Research, Vol. 29, No. 10, 10.2009, p. 3883-3886.

Research output: Contribution to journalArticle

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