C-FLIP gene silencing eliminates tumor cells in breast cancer xenografts without affecting stromal cells

Travis W. Day, Anthony L. Sinn, Su Huang, Karen E. Pollok, George E. Sandusky, Ahmad R. Safa

Research output: Contribution to journalArticle

13 Scopus citations

Abstract

Cellular FLICE-like inhibitory protein (c-FLIP) is an inhibitor of death receptor-mediated apoptosis and exerts its anti-apoptotic function by blocking the activation of caspase-8. We recently showed that the siRNA-mediated knockdown of c-FLIP in MCF-7 breast cancer cells growing in vitro triggered apoptosis. The aim of this study was to determine if the in vivo knockdown of c-FLIP in MCF-7 breast cancer xenografts affected tumor viability. Immunohistochemical detection of c-FLIP in the tumor sections revealed that the knockdown of c-FLIP eliminated the neoplastic cells within the breast cancer xenografts without affecting the normal stromal and fibroblastic cells. These results indicate that c-FLIP is required for breast cancer growth and is a relevant therapeutic target for the treatment of breast cancer.

Original languageEnglish (US)
Pages (from-to)3883-3886
Number of pages4
JournalAnticancer Research
Volume29
Issue number10
StatePublished - Oct 1 2009

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Keywords

  • Apoptosis
  • c-FLIP
  • Cancer cell xenografts
  • Immunohistochemistry

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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