c-Kit and CD38 are expressed by long-term reconstituting hematopoietic cells present in the murine yolk sac

Ramzi N. Dagher, Kelly Hiatt, Christie Traycoff, Edward E. Srour, Mervin C. Yoder

Research output: Contribution to journalArticle

22 Scopus citations

Abstract

Murine fetal liver (FL) and adult bone marrow (BM) hematopoietic stem cells (HSCs) are characterized by cell surface expression of CD38 and c-kit. Because murine yolk sac (YS) HSC activity precedes the initiation of FL hematopoiesis, we investigated whether YS-derived HSCs also expressed c-kit and CD38. c-Kit+CD38+lineage- cells derived from day 9 YS as well as adult BM were found to be enriched in high proliferative potential colony-forming cells. c-Kit+CD38+lineage- YS or adult BM cells were capable of long-term reconstitution (>6 months) of busulfan-conditioned newborn or lethally irradiated adult mice, respectively. In contrast, c-kit+CD38-lineage- populations from both tissues were enriched in lineage-committed progenitors and had no long-term HSC activity. We concluded that c-kit and CD38 are cell surface markers of HSCs expressed throughout murine ontogeny.

Original languageEnglish (US)
Pages (from-to)69-74
Number of pages6
JournalBiology of Blood and Marrow Transplantation
Volume4
Issue number2
DOIs
StatePublished - Jan 1 1998

    Fingerprint

Keywords

  • Developmental biology
  • Flow cytometry
  • Newborn transplants

ASJC Scopus subject areas

  • Transplantation

Cite this