C-MYC controlled TIP110 protein expression regulates OCT4 mRNA splicing in human embryonic stem cells

Ying Liu, Khalid Timani, Xuan Ou, Hal Broxmeyer, Johnny J. He

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

We reported previously that HIV-1 the Tat-interacting protein of 110 kDa (TIP110; P110(NRB)/SART3/p110) is important in regulation of hematopoiesis, and in maintaining pluripotent factor (NANOG, OCT4, and SOX2) expression in and pluripotency of human embryonic stem cells (hESCs). Here we show that TIP110 expression in hESCs line H9 and embryonal carcinoma cell line NT-2 is regulated by C-MYC expression in ESCs through an E box present in the TIP110 promoter region. Through up- and down- modulation of expression, TIP110 induces OCT4 exon 1a inclusion and exon 1b skipping in our OCT4 minigene model. Thus, TIP110 expression in ESCs regulates alternative splicing of OCT4, an event required for pluripotency of ESCs.

Original languageEnglish
Pages (from-to)689-694
Number of pages6
JournalStem Cells and Development
Volume22
Issue number5
DOIs
StatePublished - Mar 1 2013

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Exons
tat Gene Products
Embryonal Carcinoma Stem Cells
Cell Line
Messenger RNA
Hematopoiesis
Alternative Splicing
Genetic Promoter Regions
HIV-1
Proteins
Human Embryonic Stem Cells
methyl(arginyl)-lysyl-prolyl-tryptophyl-tert-leucyl-leucine

ASJC Scopus subject areas

  • Cell Biology
  • Developmental Biology
  • Hematology

Cite this

C-MYC controlled TIP110 protein expression regulates OCT4 mRNA splicing in human embryonic stem cells. / Liu, Ying; Timani, Khalid; Ou, Xuan; Broxmeyer, Hal; He, Johnny J.

In: Stem Cells and Development, Vol. 22, No. 5, 01.03.2013, p. 689-694.

Research output: Contribution to journalArticle

Liu, Ying ; Timani, Khalid ; Ou, Xuan ; Broxmeyer, Hal ; He, Johnny J. / C-MYC controlled TIP110 protein expression regulates OCT4 mRNA splicing in human embryonic stem cells. In: Stem Cells and Development. 2013 ; Vol. 22, No. 5. pp. 689-694.
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