C-reactive protein correlates with macrophage accumulation in coronary arteries of hypercholesterolemic pigs

James R. Turk, Jeffery A. Carroll, M. Harold Laughlin, Tom R. Thomas, Jennifer Casati, Douglas K. Bowles, Michael Sturek

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

A growing body of evidence supports the hypothesis that C-reactive protein (CRP) is a marker of inflammation in coronary artery disease. The purpose of the present study was to test the hypothesis that CRP correlates with macrophage accumulation during the initial stages of coronary vascular disease. Adult male pigs were fed a normal chow (NF) or a high-fat high-cholesterol (HF) diet for 20 wk. After 20 wk, blood was collected for analyses of interleukin-6 (IL-6), CRP, and lipids. After blood collection, the pigs were euthanized and the right coronary arteries (RCA) were harvested and fixed in neutral buffered formalin. Paraffin-embedded sections of RCA were stained immunohistochemically for CRP, scavenger receptor A (SRA), and monocyte chemoattractant protein-1 (MCP-1). All cholesterol fractions were elevated in the HF vs. the NF group (P <0.05). There Was little or no positive staining for CRP, SRA, or MCP-1 in the RCA of NF pigs, but there was extensive staining in lipid-laden macrophage foam cells in the HF pigs. Double staining revealed colocalization of CRP with SRA and CRP with MCP-1 in foam cells. Serum IL-6 was below the assay detection limit in all pigs. Serum CRP correlated directly with plasma total cholesterol (R = 0.727, P = 0.041) and accumulation of SRA-positive macrophages (R = 0.938, P <0.001) in RCA of HF pigs. We conclude that serum CRP correlates with macrophage accumulation and coronary artery disease in hypercholesterolemic pigs.

Original languageEnglish (US)
Pages (from-to)1301-1304
Number of pages4
JournalJournal of Applied Physiology
Volume95
Issue number3
StatePublished - Sep 1 2003
Externally publishedYes

Fingerprint

C-Reactive Protein
Coronary Vessels
Swine
Macrophages
Scavenger Receptors
Chemokine CCL2
Foam Cells
Cholesterol
Staining and Labeling
Blood Proteins
Coronary Artery Disease
Interleukin-6
Lipids
Vascular Diseases
Paraffin
Formaldehyde
Coronary Disease
Limit of Detection
Fats
Diet

Keywords

  • Hypercholesterolemia
  • Monocyte chemoattractant protein
  • Scavenger receptor A

ASJC Scopus subject areas

  • Physiology
  • Endocrinology
  • Orthopedics and Sports Medicine
  • Physical Therapy, Sports Therapy and Rehabilitation

Cite this

Turk, J. R., Carroll, J. A., Laughlin, M. H., Thomas, T. R., Casati, J., Bowles, D. K., & Sturek, M. (2003). C-reactive protein correlates with macrophage accumulation in coronary arteries of hypercholesterolemic pigs. Journal of Applied Physiology, 95(3), 1301-1304.

C-reactive protein correlates with macrophage accumulation in coronary arteries of hypercholesterolemic pigs. / Turk, James R.; Carroll, Jeffery A.; Laughlin, M. Harold; Thomas, Tom R.; Casati, Jennifer; Bowles, Douglas K.; Sturek, Michael.

In: Journal of Applied Physiology, Vol. 95, No. 3, 01.09.2003, p. 1301-1304.

Research output: Contribution to journalArticle

Turk, JR, Carroll, JA, Laughlin, MH, Thomas, TR, Casati, J, Bowles, DK & Sturek, M 2003, 'C-reactive protein correlates with macrophage accumulation in coronary arteries of hypercholesterolemic pigs', Journal of Applied Physiology, vol. 95, no. 3, pp. 1301-1304.
Turk JR, Carroll JA, Laughlin MH, Thomas TR, Casati J, Bowles DK et al. C-reactive protein correlates with macrophage accumulation in coronary arteries of hypercholesterolemic pigs. Journal of Applied Physiology. 2003 Sep 1;95(3):1301-1304.
Turk, James R. ; Carroll, Jeffery A. ; Laughlin, M. Harold ; Thomas, Tom R. ; Casati, Jennifer ; Bowles, Douglas K. ; Sturek, Michael. / C-reactive protein correlates with macrophage accumulation in coronary arteries of hypercholesterolemic pigs. In: Journal of Applied Physiology. 2003 ; Vol. 95, No. 3. pp. 1301-1304.
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