Ca2+-permeable large-conductance nonselective cation channels in rat basophilic leukemia cells

Alexander Obukhov, S. V P Jones, V. E. Degtiar, A. Luckhoff, G. Schultz, J. Hescheler

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Spreading of Ca2+ signals in rat basophilic leukemia (RBL) cells occurs by release of ATP. Therefore we studied the effect of ATP on membrane currents. ATP (1-10 μM) activated large-conductance channels. Single channel events were resolved in the whole cell mode. Similar channel activity was observed in RBL cells transfected with the muscarinic M1 receptor after stimulation with carbachol as well as after intracellular infusion of aluminum fluoride. Activation was independent of internal Ca2+ (0-10 μM). The channels had a conductance of 250 pS in 135 mM Na+ and 70 pS in 100 mM Ca2+. The permeability (P) ratio was P(Ca)/P(Na)/P(Cs)/P(Mg) = 16:1:0.6:0.6. These channels may contribute to secretory responses by allowing Ca2+ entry, leading to high Ca2+ concentrations in the vicinity of the channel pore.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Cell Physiology
Volume269
Issue number5 38-5
StatePublished - 1995
Externally publishedYes

Fingerprint

leukemia
Cations
Rats
cations
Leukemia
Adenosine Triphosphate
calcium
rats
Muscarinic M1 Receptors
Carbachol
cells
Permeability
carbachol
Chemical activation
Membranes
fluorides
aluminum
permeability
receptors

Keywords

  • muscarinic M receptor
  • nonselective cation channels
  • patch clamp
  • purinoceptors
  • RBL-2H3 cells

ASJC Scopus subject areas

  • Cell Biology
  • Clinical Biochemistry
  • Physiology
  • Agricultural and Biological Sciences(all)

Cite this

Obukhov, A., Jones, S. V. P., Degtiar, V. E., Luckhoff, A., Schultz, G., & Hescheler, J. (1995). Ca2+-permeable large-conductance nonselective cation channels in rat basophilic leukemia cells. American Journal of Physiology - Cell Physiology, 269(5 38-5).

Ca2+-permeable large-conductance nonselective cation channels in rat basophilic leukemia cells. / Obukhov, Alexander; Jones, S. V P; Degtiar, V. E.; Luckhoff, A.; Schultz, G.; Hescheler, J.

In: American Journal of Physiology - Cell Physiology, Vol. 269, No. 5 38-5, 1995.

Research output: Contribution to journalArticle

Obukhov, Alexander ; Jones, S. V P ; Degtiar, V. E. ; Luckhoff, A. ; Schultz, G. ; Hescheler, J. / Ca2+-permeable large-conductance nonselective cation channels in rat basophilic leukemia cells. In: American Journal of Physiology - Cell Physiology. 1995 ; Vol. 269, No. 5 38-5.
@article{5340d2823d8b480ead021e0bda59752e,
title = "Ca2+-permeable large-conductance nonselective cation channels in rat basophilic leukemia cells",
abstract = "Spreading of Ca2+ signals in rat basophilic leukemia (RBL) cells occurs by release of ATP. Therefore we studied the effect of ATP on membrane currents. ATP (1-10 μM) activated large-conductance channels. Single channel events were resolved in the whole cell mode. Similar channel activity was observed in RBL cells transfected with the muscarinic M1 receptor after stimulation with carbachol as well as after intracellular infusion of aluminum fluoride. Activation was independent of internal Ca2+ (0-10 μM). The channels had a conductance of 250 pS in 135 mM Na+ and 70 pS in 100 mM Ca2+. The permeability (P) ratio was P(Ca)/P(Na)/P(Cs)/P(Mg) = 16:1:0.6:0.6. These channels may contribute to secretory responses by allowing Ca2+ entry, leading to high Ca2+ concentrations in the vicinity of the channel pore.",
keywords = "muscarinic M receptor, nonselective cation channels, patch clamp, purinoceptors, RBL-2H3 cells",
author = "Alexander Obukhov and Jones, {S. V P} and Degtiar, {V. E.} and A. Luckhoff and G. Schultz and J. Hescheler",
year = "1995",
language = "English (US)",
volume = "269",
journal = "American Journal of Physiology",
issn = "0193-1857",
publisher = "American Physiological Society",
number = "5 38-5",

}

TY - JOUR

T1 - Ca2+-permeable large-conductance nonselective cation channels in rat basophilic leukemia cells

AU - Obukhov, Alexander

AU - Jones, S. V P

AU - Degtiar, V. E.

AU - Luckhoff, A.

AU - Schultz, G.

AU - Hescheler, J.

PY - 1995

Y1 - 1995

N2 - Spreading of Ca2+ signals in rat basophilic leukemia (RBL) cells occurs by release of ATP. Therefore we studied the effect of ATP on membrane currents. ATP (1-10 μM) activated large-conductance channels. Single channel events were resolved in the whole cell mode. Similar channel activity was observed in RBL cells transfected with the muscarinic M1 receptor after stimulation with carbachol as well as after intracellular infusion of aluminum fluoride. Activation was independent of internal Ca2+ (0-10 μM). The channels had a conductance of 250 pS in 135 mM Na+ and 70 pS in 100 mM Ca2+. The permeability (P) ratio was P(Ca)/P(Na)/P(Cs)/P(Mg) = 16:1:0.6:0.6. These channels may contribute to secretory responses by allowing Ca2+ entry, leading to high Ca2+ concentrations in the vicinity of the channel pore.

AB - Spreading of Ca2+ signals in rat basophilic leukemia (RBL) cells occurs by release of ATP. Therefore we studied the effect of ATP on membrane currents. ATP (1-10 μM) activated large-conductance channels. Single channel events were resolved in the whole cell mode. Similar channel activity was observed in RBL cells transfected with the muscarinic M1 receptor after stimulation with carbachol as well as after intracellular infusion of aluminum fluoride. Activation was independent of internal Ca2+ (0-10 μM). The channels had a conductance of 250 pS in 135 mM Na+ and 70 pS in 100 mM Ca2+. The permeability (P) ratio was P(Ca)/P(Na)/P(Cs)/P(Mg) = 16:1:0.6:0.6. These channels may contribute to secretory responses by allowing Ca2+ entry, leading to high Ca2+ concentrations in the vicinity of the channel pore.

KW - muscarinic M receptor

KW - nonselective cation channels

KW - patch clamp

KW - purinoceptors

KW - RBL-2H3 cells

UR - http://www.scopus.com/inward/record.url?scp=0028865047&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028865047&partnerID=8YFLogxK

M3 - Article

C2 - 7491899

AN - SCOPUS:0028865047

VL - 269

JO - American Journal of Physiology

JF - American Journal of Physiology

SN - 0193-1857

IS - 5 38-5

ER -