Ca2+-transporting ATPase, phospholamban, and caisequestrin levels in nonfailing and failing human myocardium

Matthew A. Movsesian, Mohsen Karimi, Karen Green, Larry Jones

Research output: Contribution to journalArticle

204 Citations (Scopus)

Abstract

Background: Observations of abnormalities in the diastolic components of intracellular Ca2+ transients in failing human left ventricular myocardium have raised the possibility that reductions in the level or function of sarcoplasmic reticulum proteins involved in Ca2+ transport contribute to the pathophysiology of dilated cardiomyopathy in humans. Functional assays, however, have revealed no differences in ATP-dependent Ca2+ transport or its modulation by phospholamban in sarcoplasmic reticulum-enriched microsomes prepared from nonfailing and failing human left ventricular myocardium. The purpose of the present study was to quantify protein levels of Ca2+-transporting ATPase, phospholamban, and calsequestrin directly in nonfailing and failing human left ventricular myocardium. Methods and Results: Total protein extracts were prepared from nonfailing left ventricular myocardium from the hearts of unmatched organ donors with normal left ventricular contractility (n=6) and from failing left ventricular myocardium from the excised hearts of transplant recipients with class IV heart failure resulting from idiopathic dilated cardiomyopathy (n=6). Ca2+-transporting ATPase, phospholamban, and calsequestrin contents were determined by quantitative immunoblotting with monoclonal and affinity-purified polyclonal antibodies. The levels of the three proteins were identical in nonfailing and failing human left ventricular myocardium. Conclusions: These results indicate that protein levels of Ca2+-transporting ATPase, phospholamban, and calsequestrin are not diminished in failing human left ventricular myocardium and that downregulation of the Ca2+-transporting ATPase and phospholamban is not part of the molecular pathophysiology of dilated cardiomyopathy in humans.

Original languageEnglish
Pages (from-to)653-657
Number of pages5
JournalCirculation
Volume90
Issue number2
StatePublished - Aug 1994

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Calcium-Transporting ATPases
Myocardium
Calsequestrin
Dilated Cardiomyopathy
Sarcoplasmic Reticulum
Proteins
Microsomes
phospholamban
Immunoblotting
Down-Regulation
Heart Failure
Adenosine Triphosphate
Tissue Donors
Antibodies

Keywords

  • Ca ATPase
  • Calsequestrin
  • Cardiomyopathy
  • Phospholamban

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine

Cite this

Ca2+-transporting ATPase, phospholamban, and caisequestrin levels in nonfailing and failing human myocardium. / Movsesian, Matthew A.; Karimi, Mohsen; Green, Karen; Jones, Larry.

In: Circulation, Vol. 90, No. 2, 08.1994, p. 653-657.

Research output: Contribution to journalArticle

Movsesian, Matthew A. ; Karimi, Mohsen ; Green, Karen ; Jones, Larry. / Ca2+-transporting ATPase, phospholamban, and caisequestrin levels in nonfailing and failing human myocardium. In: Circulation. 1994 ; Vol. 90, No. 2. pp. 653-657.
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