Cafeteria diet is a robust model of human metabolic syndrome with liver and adipose inflammation: Comparison to high-fat diet

Brante P. Sampey, Amanda M. Vanhoose, Helena M. Winfield, Alex J. Freemerman, Michael J. Muehlbauer, Patrick T. Fueger, Christopher B. Newgard, Liza Makowski

Research output: Contribution to journalArticle

262 Citations (Scopus)

Abstract

Obesity has reached epidemic proportions worldwide and reports estimate that American children consume up to 25% of calories from snacks. Several animal models of obesity exist, but studies are lacking that compare high-fat diets (HFD) traditionally used in rodent models of diet-induced obesity (DIO) to diets consisting of food regularly consumed by humans, including high-salt, high-fat, low-fiber, energy dense foods such as cookies, chips, and processed meats. To investigate the obesogenic and inflammatory consequences of a cafeteria diet (CAF) compared to a lard-based 45% HFD in rodent models, male Wistar rats were fed HFD, CAF or chow control diets for 15 weeks. Body weight increased dramatically and remained significantly elevated in CAF-fed rats compared to all other diets. Glucose- and insulin-tolerance tests revealed that hyperinsulinemia, hyperglycemia, and glucose intolerance were exaggerated in the CAF-fed rats compared to controls and HFD-fed rats. It is well-established that macrophages infiltrate metabolic tissues at the onset of weight gain and directly contribute to inflammation, insulin resistance, and obesity. Although both high fat diets resulted in increased adiposity and hepatosteatosis, CAF-fed rats displayed remarkable inflammation in white fat, brown fat and liver compared to HFD and controls. In sum, the CAF provided a robust model of human metabolic syndrome compared to traditional lard-based HFD, creating a phenotype of exaggerated obesity with glucose intolerance and inflammation. This model provides a unique platform to study the biochemical, genomic and physiological mechanisms of obesity and obesity-related disease states that are pandemic in western civilization today.

Original languageEnglish
Pages (from-to)1109-1117
Number of pages9
JournalObesity
Volume19
Issue number6
DOIs
StatePublished - Jun 2011

Fingerprint

High Fat Diet
Diet
Inflammation
Liver
Obesity
Glucose Intolerance
Rodentia
Civilization
Food
White Adipose Tissue
Snacks
Brown Adipose Tissue
Adiposity
Hyperinsulinism
Pandemics
Glucose Tolerance Test
Hyperglycemia
Meat
Weight Gain
Insulin Resistance

ASJC Scopus subject areas

  • Endocrinology
  • Medicine (miscellaneous)
  • Endocrinology, Diabetes and Metabolism
  • Nutrition and Dietetics

Cite this

Sampey, B. P., Vanhoose, A. M., Winfield, H. M., Freemerman, A. J., Muehlbauer, M. J., Fueger, P. T., ... Makowski, L. (2011). Cafeteria diet is a robust model of human metabolic syndrome with liver and adipose inflammation: Comparison to high-fat diet. Obesity, 19(6), 1109-1117. https://doi.org/10.1038/oby.2011.18

Cafeteria diet is a robust model of human metabolic syndrome with liver and adipose inflammation : Comparison to high-fat diet. / Sampey, Brante P.; Vanhoose, Amanda M.; Winfield, Helena M.; Freemerman, Alex J.; Muehlbauer, Michael J.; Fueger, Patrick T.; Newgard, Christopher B.; Makowski, Liza.

In: Obesity, Vol. 19, No. 6, 06.2011, p. 1109-1117.

Research output: Contribution to journalArticle

Sampey, BP, Vanhoose, AM, Winfield, HM, Freemerman, AJ, Muehlbauer, MJ, Fueger, PT, Newgard, CB & Makowski, L 2011, 'Cafeteria diet is a robust model of human metabolic syndrome with liver and adipose inflammation: Comparison to high-fat diet', Obesity, vol. 19, no. 6, pp. 1109-1117. https://doi.org/10.1038/oby.2011.18
Sampey BP, Vanhoose AM, Winfield HM, Freemerman AJ, Muehlbauer MJ, Fueger PT et al. Cafeteria diet is a robust model of human metabolic syndrome with liver and adipose inflammation: Comparison to high-fat diet. Obesity. 2011 Jun;19(6):1109-1117. https://doi.org/10.1038/oby.2011.18
Sampey, Brante P. ; Vanhoose, Amanda M. ; Winfield, Helena M. ; Freemerman, Alex J. ; Muehlbauer, Michael J. ; Fueger, Patrick T. ; Newgard, Christopher B. ; Makowski, Liza. / Cafeteria diet is a robust model of human metabolic syndrome with liver and adipose inflammation : Comparison to high-fat diet. In: Obesity. 2011 ; Vol. 19, No. 6. pp. 1109-1117.
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