Calcium and vitamin D intake maintained from preovariectomy independently affect calcium metabolism and bone properties in Sprague Dawley rats

C. Y. Park, W. H. Lee, J. C. Fleet, Matthew Allen, G. P. McCabe, D. M. Walsh, C. M. Weaver

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The interaction of habitual Ca and vitamin D intake from preovariectomy to 4 months postovariectomy on bone and Ca metabolism was assessed. Higher Ca intake suppressed net bone turnover, and both nutrients independently benefitted trabecular structure. Habitual intake of adequate Ca and ~50 nmol/L vitamin D status is most beneficial. Introduction: Dietary strategies to benefit bone are typically tested prior to or after menopause but not through menopause transition. We investigated the interaction of Ca and vitamin D status on Ca absorption, bone remodeling, Ca kinetics, and bone strength as rats transitioned through estrogen deficiency. Methods: Sprague Dawley rats were randomized at 8 weeks to 0.2 or 1.0 % Ca and 50, 100, or 1,000 IU (1.25, 2.5, or 25 μg) vitamin D/kg diet (2∈×∈3 factorial design) and ovariectomized at 12 weeks. Urinary 45Ca excretion from deep-labeled bone was used to assess net bone turnover weekly. Ca kinetics was performed between 25 and 28 weeks. Rats were killed at 29 weeks. Femoral and tibiae structure (by μCT), dynamic histomorphometry, and bone Ca content were assessed. Results: Mean 25(OH)D for rats on the 50, 100, 1,000 IU vitamin D/kg diet were 32, 54, and 175 nmol/L, respectively. Higher Ca intake ameliorated net bone turnover, reduced fractional Ca absorption and bone resorption, and increased net Ca absorption. Tibial and femoral trabecular structures were enhanced independently by higher Ca and vitamin D intake. Tibial bone width and fracture resistance were enhanced by higher vitamin D intake. Dynamic histomorphometry in the tibia was not affected by either nutrient. A Ca × vitamin D interaction existed in femur length, tibial Ca content, and mass of the soft tissue/extracellular fluid compartment. Conclusions: Adequate Ca intake and serum 25(OH)D level of 50 nmol/L provided the most benefit for bone health, mostly through independent effects of Ca and vitamin D.

Original languageEnglish
Pages (from-to)1905-1915
Number of pages11
JournalOsteoporosis International
Volume25
Issue number7
DOIs
StatePublished - 2014

Fingerprint

Vitamin D
Sprague Dawley Rats
Calcium
Bone and Bones
Bone Remodeling
Menopause
Thigh
Tibia
Diet
Food
Bone Fractures
Extracellular Fluid
Insurance Benefits
Bone Resorption
Femur
Estrogens
Serum

Keywords

  • Calcium
  • Histomorphometry
  • Kinetics
  • Ovariectomy
  • Vitamin D

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Medicine(all)

Cite this

Calcium and vitamin D intake maintained from preovariectomy independently affect calcium metabolism and bone properties in Sprague Dawley rats. / Park, C. Y.; Lee, W. H.; Fleet, J. C.; Allen, Matthew; McCabe, G. P.; Walsh, D. M.; Weaver, C. M.

In: Osteoporosis International, Vol. 25, No. 7, 2014, p. 1905-1915.

Research output: Contribution to journalArticle

Park, C. Y. ; Lee, W. H. ; Fleet, J. C. ; Allen, Matthew ; McCabe, G. P. ; Walsh, D. M. ; Weaver, C. M. / Calcium and vitamin D intake maintained from preovariectomy independently affect calcium metabolism and bone properties in Sprague Dawley rats. In: Osteoporosis International. 2014 ; Vol. 25, No. 7. pp. 1905-1915.
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T1 - Calcium and vitamin D intake maintained from preovariectomy independently affect calcium metabolism and bone properties in Sprague Dawley rats

AU - Park, C. Y.

AU - Lee, W. H.

AU - Fleet, J. C.

AU - Allen, Matthew

AU - McCabe, G. P.

AU - Walsh, D. M.

AU - Weaver, C. M.

PY - 2014

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AB - The interaction of habitual Ca and vitamin D intake from preovariectomy to 4 months postovariectomy on bone and Ca metabolism was assessed. Higher Ca intake suppressed net bone turnover, and both nutrients independently benefitted trabecular structure. Habitual intake of adequate Ca and ~50 nmol/L vitamin D status is most beneficial. Introduction: Dietary strategies to benefit bone are typically tested prior to or after menopause but not through menopause transition. We investigated the interaction of Ca and vitamin D status on Ca absorption, bone remodeling, Ca kinetics, and bone strength as rats transitioned through estrogen deficiency. Methods: Sprague Dawley rats were randomized at 8 weeks to 0.2 or 1.0 % Ca and 50, 100, or 1,000 IU (1.25, 2.5, or 25 μg) vitamin D/kg diet (2∈×∈3 factorial design) and ovariectomized at 12 weeks. Urinary 45Ca excretion from deep-labeled bone was used to assess net bone turnover weekly. Ca kinetics was performed between 25 and 28 weeks. Rats were killed at 29 weeks. Femoral and tibiae structure (by μCT), dynamic histomorphometry, and bone Ca content were assessed. Results: Mean 25(OH)D for rats on the 50, 100, 1,000 IU vitamin D/kg diet were 32, 54, and 175 nmol/L, respectively. Higher Ca intake ameliorated net bone turnover, reduced fractional Ca absorption and bone resorption, and increased net Ca absorption. Tibial and femoral trabecular structures were enhanced independently by higher Ca and vitamin D intake. Tibial bone width and fracture resistance were enhanced by higher vitamin D intake. Dynamic histomorphometry in the tibia was not affected by either nutrient. A Ca × vitamin D interaction existed in femur length, tibial Ca content, and mass of the soft tissue/extracellular fluid compartment. Conclusions: Adequate Ca intake and serum 25(OH)D level of 50 nmol/L provided the most benefit for bone health, mostly through independent effects of Ca and vitamin D.

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KW - Kinetics

KW - Ovariectomy

KW - Vitamin D

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