Calcium Stoichiometry Determination for Calcium Binding Proteins by Electrospray Ionization Mass Spectrometry

Peifeng Hu, Qi Zhuang Ye, Joseph A. Loo

Research output: Contribution to journalArticle

110 Scopus citations

Abstract

Electrospray ionization mass spectrometry was used for the determination of calcium-binding stoichiometry for calcium-binding proteins. Bovine calmodulin, bovine α-lactalbumin, and rabbit parvalbumin were found to bind specifically to 4, 1, and 2 Ca2+ ions, respectively, in agreement with previously reported results obtained by using other physical methods. This mass spectrometry method could also be applied to proteins that bind more than one type of metal ion. The Zn2+- and Ca2+-binding stoichiometries for human stromelysin catalytic domain were determined to be 3 and 2, respectively.

Original languageEnglish (US)
Pages (from-to)4190-4194
Number of pages5
JournalAnalytical Chemistry
Volume66
Issue number23
DOIs
StatePublished - Dec 1 1994
Externally publishedYes

ASJC Scopus subject areas

  • Analytical Chemistry

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