Calcium Stoichiometry Determination for Calcium Binding Proteins by Electrospray Ionization Mass Spectrometry

Peifeng Hu, Qi Zhuang Ye, Joseph A. Loo

Research output: Contribution to journalArticle

109 Citations (Scopus)

Abstract

Electrospray ionization mass spectrometry was used for the determination of calcium-binding stoichiometry for calcium-binding proteins. Bovine calmodulin, bovine α-lactalbumin, and rabbit parvalbumin were found to bind specifically to 4, 1, and 2 Ca2+ ions, respectively, in agreement with previously reported results obtained by using other physical methods. This mass spectrometry method could also be applied to proteins that bind more than one type of metal ion. The Zn2+- and Ca2+-binding stoichiometries for human stromelysin catalytic domain were determined to be 3 and 2, respectively.

Original languageEnglish (US)
Pages (from-to)4190-4194
Number of pages5
JournalAnalytical Chemistry
Volume66
Issue number23
DOIs
StatePublished - Dec 1 1994

Fingerprint

Electrospray ionization
Calcium-Binding Proteins
Stoichiometry
Mass spectrometry
Calcium
Lactalbumin
Parvalbumins
Matrix Metalloproteinase 3
Calmodulin
Metal ions
Ions
Proteins

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Calcium Stoichiometry Determination for Calcium Binding Proteins by Electrospray Ionization Mass Spectrometry. / Hu, Peifeng; Ye, Qi Zhuang; Loo, Joseph A.

In: Analytical Chemistry, Vol. 66, No. 23, 01.12.1994, p. 4190-4194.

Research output: Contribution to journalArticle

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