Calprotectin Increases the Activity of the SaeRS Two Component System and Murine Mortality during Staphylococcus aureus Infections

Hoonsik Cho, Do Won Jeong, Qian Liu, Won Sik Yeo, Thomas Vogl, Eric P. Skaar, Walter J. Chazin, Taeok Bae

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Calprotectin, the most abundant cytoplasmic protein in neutrophils, suppresses the growth of Staphylococcus aureus by sequestering the nutrient metal ions Zn and Mn. Here we show that calprotectin can also enhance the activity of the SaeRS two component system (TCS), a signaling system essential for production of over 20 virulence factors in S. aureus. The activity of the SaeRS TCS is repressed by certain divalent ions found in blood or neutrophil granules; however, the Zn bound-form of calprotectin relieves this repression. During staphylococcal encounter with murine neutrophils or staphylococcal infection of the murine peritoneal cavity, calprotectin increases the activity of the SaeRS TCS as well as the production of proinflammatory cytokines such as IL-1β and TNF-α, resulting in higher murine mortality. These results suggest that, under certain conditions, calprotectin can be exploited by S. aureus to increase bacterial virulence and host mortality.

Original languageEnglish (US)
Article numbere1005026
JournalPLoS Pathogens
Volume11
Issue number7
DOIs
StatePublished - Jul 1 2015

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Leukocyte L1 Antigen Complex
Staphylococcus aureus
Mortality
Infection
Neutrophils
Ions
Staphylococcal Infections
Peritoneal Cavity
Virulence Factors
Interleukin-1
Virulence
Metals
Cytokines
Food
Growth
Proteins

ASJC Scopus subject areas

  • Microbiology
  • Parasitology
  • Virology
  • Immunology
  • Genetics
  • Molecular Biology

Cite this

Calprotectin Increases the Activity of the SaeRS Two Component System and Murine Mortality during Staphylococcus aureus Infections. / Cho, Hoonsik; Jeong, Do Won; Liu, Qian; Yeo, Won Sik; Vogl, Thomas; Skaar, Eric P.; Chazin, Walter J.; Bae, Taeok.

In: PLoS Pathogens, Vol. 11, No. 7, e1005026, 01.07.2015.

Research output: Contribution to journalArticle

Cho, Hoonsik ; Jeong, Do Won ; Liu, Qian ; Yeo, Won Sik ; Vogl, Thomas ; Skaar, Eric P. ; Chazin, Walter J. ; Bae, Taeok. / Calprotectin Increases the Activity of the SaeRS Two Component System and Murine Mortality during Staphylococcus aureus Infections. In: PLoS Pathogens. 2015 ; Vol. 11, No. 7.
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