Can GEWF solution improve the retrieval of lymph nodes from colorectal cancer resections?

Steven F. Gregurek, Howard Wu

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Context.-Lymph node status is an important prognostic factor in colorectal cancer staging. Objective.-To determine whether the use of GEWF solution (glacial acetic acid, ethanol, distilled water, formaldehyde) increases the yield of lymph nodes (LNs) from colorectal cancer specimens. Design.-A prospective study investigated the usefulness of GEWF in identifying LN from colorectal cancer resection specimens. After standard formalin fixation, approximately half of the specimens were placed in GEWF solution, and the remainder were used as a control. The number of LNs, patient sex, length of specimen, site of the specimen, tumor staging, and the number of positive LNs were analyzed. The numbers of retrieved and metastasis-positive LNs were recorded and compared with specimens from the previous year, in which no GEWF solution was used. Residents were educated on the importance of LN status before the study. Data were analyzed using independent-sample t tests. The residents also completed a subjective survey at the conclusion of the study. Results.-A total of 85 specimens, 45 (53%) with GEWF and 40 (47%) without GEWF, were examined by 11 residents. The mean number of retrieved LNs using GEWF was slightly higher than the control group (19.96 vs 18.30); however, there was no statistical significance (P = .53). The mean number of LNs from the current study (2004-2005) is significantly higher than that from the previous year (2003-2004) (19.17 vs 11.51, P <.001). Conclusions.-GEWF did not increase the yield of LNs from colorectal cancer specimens. However, application of the guideline for a minimum of 12 LNs significantly increased the LN retrieval.

Original languageEnglish (US)
Pages (from-to)83-86
Number of pages4
JournalArchives of Pathology and Laboratory Medicine
Volume133
Issue number1
StatePublished - Jan 2009
Externally publishedYes

Fingerprint

Colorectal Neoplasms
Lymph Nodes
Neoplasm Staging
Formaldehyde
Acetic Acid
Ethanol
Prospective Studies
Guidelines
Neoplasm Metastasis
Control Groups
Water

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Medical Laboratory Technology

Cite this

Can GEWF solution improve the retrieval of lymph nodes from colorectal cancer resections? / Gregurek, Steven F.; Wu, Howard.

In: Archives of Pathology and Laboratory Medicine, Vol. 133, No. 1, 01.2009, p. 83-86.

Research output: Contribution to journalArticle

@article{cff727dfcdbd430a98b22fec7f460cbd,
title = "Can GEWF solution improve the retrieval of lymph nodes from colorectal cancer resections?",
abstract = "Context.-Lymph node status is an important prognostic factor in colorectal cancer staging. Objective.-To determine whether the use of GEWF solution (glacial acetic acid, ethanol, distilled water, formaldehyde) increases the yield of lymph nodes (LNs) from colorectal cancer specimens. Design.-A prospective study investigated the usefulness of GEWF in identifying LN from colorectal cancer resection specimens. After standard formalin fixation, approximately half of the specimens were placed in GEWF solution, and the remainder were used as a control. The number of LNs, patient sex, length of specimen, site of the specimen, tumor staging, and the number of positive LNs were analyzed. The numbers of retrieved and metastasis-positive LNs were recorded and compared with specimens from the previous year, in which no GEWF solution was used. Residents were educated on the importance of LN status before the study. Data were analyzed using independent-sample t tests. The residents also completed a subjective survey at the conclusion of the study. Results.-A total of 85 specimens, 45 (53{\%}) with GEWF and 40 (47{\%}) without GEWF, were examined by 11 residents. The mean number of retrieved LNs using GEWF was slightly higher than the control group (19.96 vs 18.30); however, there was no statistical significance (P = .53). The mean number of LNs from the current study (2004-2005) is significantly higher than that from the previous year (2003-2004) (19.17 vs 11.51, P <.001). Conclusions.-GEWF did not increase the yield of LNs from colorectal cancer specimens. However, application of the guideline for a minimum of 12 LNs significantly increased the LN retrieval.",
author = "Gregurek, {Steven F.} and Howard Wu",
year = "2009",
month = "1",
language = "English (US)",
volume = "133",
pages = "83--86",
journal = "Archives of Pathology and Laboratory Medicine",
issn = "0003-9985",
publisher = "College of American Pathologists",
number = "1",

}

TY - JOUR

T1 - Can GEWF solution improve the retrieval of lymph nodes from colorectal cancer resections?

AU - Gregurek, Steven F.

AU - Wu, Howard

PY - 2009/1

Y1 - 2009/1

N2 - Context.-Lymph node status is an important prognostic factor in colorectal cancer staging. Objective.-To determine whether the use of GEWF solution (glacial acetic acid, ethanol, distilled water, formaldehyde) increases the yield of lymph nodes (LNs) from colorectal cancer specimens. Design.-A prospective study investigated the usefulness of GEWF in identifying LN from colorectal cancer resection specimens. After standard formalin fixation, approximately half of the specimens were placed in GEWF solution, and the remainder were used as a control. The number of LNs, patient sex, length of specimen, site of the specimen, tumor staging, and the number of positive LNs were analyzed. The numbers of retrieved and metastasis-positive LNs were recorded and compared with specimens from the previous year, in which no GEWF solution was used. Residents were educated on the importance of LN status before the study. Data were analyzed using independent-sample t tests. The residents also completed a subjective survey at the conclusion of the study. Results.-A total of 85 specimens, 45 (53%) with GEWF and 40 (47%) without GEWF, were examined by 11 residents. The mean number of retrieved LNs using GEWF was slightly higher than the control group (19.96 vs 18.30); however, there was no statistical significance (P = .53). The mean number of LNs from the current study (2004-2005) is significantly higher than that from the previous year (2003-2004) (19.17 vs 11.51, P <.001). Conclusions.-GEWF did not increase the yield of LNs from colorectal cancer specimens. However, application of the guideline for a minimum of 12 LNs significantly increased the LN retrieval.

AB - Context.-Lymph node status is an important prognostic factor in colorectal cancer staging. Objective.-To determine whether the use of GEWF solution (glacial acetic acid, ethanol, distilled water, formaldehyde) increases the yield of lymph nodes (LNs) from colorectal cancer specimens. Design.-A prospective study investigated the usefulness of GEWF in identifying LN from colorectal cancer resection specimens. After standard formalin fixation, approximately half of the specimens were placed in GEWF solution, and the remainder were used as a control. The number of LNs, patient sex, length of specimen, site of the specimen, tumor staging, and the number of positive LNs were analyzed. The numbers of retrieved and metastasis-positive LNs were recorded and compared with specimens from the previous year, in which no GEWF solution was used. Residents were educated on the importance of LN status before the study. Data were analyzed using independent-sample t tests. The residents also completed a subjective survey at the conclusion of the study. Results.-A total of 85 specimens, 45 (53%) with GEWF and 40 (47%) without GEWF, were examined by 11 residents. The mean number of retrieved LNs using GEWF was slightly higher than the control group (19.96 vs 18.30); however, there was no statistical significance (P = .53). The mean number of LNs from the current study (2004-2005) is significantly higher than that from the previous year (2003-2004) (19.17 vs 11.51, P <.001). Conclusions.-GEWF did not increase the yield of LNs from colorectal cancer specimens. However, application of the guideline for a minimum of 12 LNs significantly increased the LN retrieval.

UR - http://www.scopus.com/inward/record.url?scp=58349110991&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=58349110991&partnerID=8YFLogxK

M3 - Article

C2 - 19123742

AN - SCOPUS:58349110991

VL - 133

SP - 83

EP - 86

JO - Archives of Pathology and Laboratory Medicine

JF - Archives of Pathology and Laboratory Medicine

SN - 0003-9985

IS - 1

ER -