Cancer affects microRNA expression, release, and function in cardiac and skeletal muscle

Daohong Chen, Chirayu P. Goswami, Riesa M. Burnett, Manjushree Anjanappa, Poornima Bhat-Nakshatri, William Muller, Harikrishna Nakshatri

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Circulating microRNAs (miRNA) are emerging as important biomarkers of various diseases, including cancer. Intriguingly, circulating levels of several miRNAs are lower in patients with cancer compared with healthy individuals. In this study, we tested the hypothesis that a circulating miRNA might serve as a surrogate of the effects of cancer on miRNA expression or release in distant organs. Here we report that circulating levels of the muscle-enriched miR486 is lower in patients with breast cancer compared with healthy individuals and that this difference is replicated faithfully in MMTV-PyMT and MMTV-Her2 transgenic mouse models of breast cancer. In tumor-bearing mice, levels of miR486 were relatively reduced in muscle, where there was elevated expression of the miR486 target genes PTEN and FOXO1A and dampened signaling through the PI3K/AKT pathway. Skeletal muscle expressed lower levels of the transcription factor MyoD, which controls miR486 expression. Conditioned media (CM) obtained from MMTV-PyMT and MMTV-Her2/Neu tumor cells cultured in vitro were sufficient to elicit reduced levels of miR486 and increased PTEN and FOXO1A expression in C2C12 murine myoblasts. Cytokine analysis implicated tumor necrosis factor α (TNFα) and four additional cytokines as mediators of miR486 expression in CM-treated cells. Because miR486 is a potent modulator of PI3K/AKT signaling and the muscle-enriched transcription factor network in cardiac/skeletal muscle, our findings implicated TNFα-dependent miRNA circuitry in muscle differentiation and survival pathways in cancer.

Original languageEnglish (US)
Pages (from-to)4270-4281
Number of pages12
JournalCancer Research
Volume74
Issue number16
DOIs
StatePublished - Aug 15 2014

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MicroRNAs
Myocardium
Skeletal Muscle
Muscles
Neoplasms
Conditioned Culture Medium
Phosphatidylinositol 3-Kinases
Transcription Factors
Tumor Necrosis Factor-alpha
Breast Neoplasms
Cytokines
Cultured Tumor Cells
Myoblasts
Individuality
Transgenic Mice
Biomarkers
Survival
Genes

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Chen, D., Goswami, C. P., Burnett, R. M., Anjanappa, M., Bhat-Nakshatri, P., Muller, W., & Nakshatri, H. (2014). Cancer affects microRNA expression, release, and function in cardiac and skeletal muscle. Cancer Research, 74(16), 4270-4281. https://doi.org/10.1158/0008-5472.CAN-13-2817

Cancer affects microRNA expression, release, and function in cardiac and skeletal muscle. / Chen, Daohong; Goswami, Chirayu P.; Burnett, Riesa M.; Anjanappa, Manjushree; Bhat-Nakshatri, Poornima; Muller, William; Nakshatri, Harikrishna.

In: Cancer Research, Vol. 74, No. 16, 15.08.2014, p. 4270-4281.

Research output: Contribution to journalArticle

Chen, D, Goswami, CP, Burnett, RM, Anjanappa, M, Bhat-Nakshatri, P, Muller, W & Nakshatri, H 2014, 'Cancer affects microRNA expression, release, and function in cardiac and skeletal muscle', Cancer Research, vol. 74, no. 16, pp. 4270-4281. https://doi.org/10.1158/0008-5472.CAN-13-2817
Chen D, Goswami CP, Burnett RM, Anjanappa M, Bhat-Nakshatri P, Muller W et al. Cancer affects microRNA expression, release, and function in cardiac and skeletal muscle. Cancer Research. 2014 Aug 15;74(16):4270-4281. https://doi.org/10.1158/0008-5472.CAN-13-2817
Chen, Daohong ; Goswami, Chirayu P. ; Burnett, Riesa M. ; Anjanappa, Manjushree ; Bhat-Nakshatri, Poornima ; Muller, William ; Nakshatri, Harikrishna. / Cancer affects microRNA expression, release, and function in cardiac and skeletal muscle. In: Cancer Research. 2014 ; Vol. 74, No. 16. pp. 4270-4281.
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