Abstract
Invasion and metastasis of cancer cells is a complex process requiring the activity of proteins that promote extracellular matrix degradation, motility of cancer cells, and angiogenesis. Although exclusively the cancer cells make several of these proteins, few key proteins are derived from stromal cells in response to cancer cell-stromal cell interaction. In this report, we show that the breast cancer cell-derived interleukin-1alpha (IL-1α) plays an important role in expression of pro-metastatic genes in cancer as well as in stromal cells. Neutralizing antibody against IL-1α inhibited IL-6, and IL-8 expression in IL-1α-expressing cancer cells. In addition, this antibody also prevented induction of IL-6, IL-8, and matrix metalloproteinase 3 (MMP3) but not vascular endothelial growth factor (VEGF) in fibroblasts by conditioned medium (CM) from IL-1α-expressing breast cancer cells. These resuits suggest that inhibition of IL-1α activity by either neutralizing antibody against IL-1α or chemical inhibitor of IL-1α processing may prevent invasion and metastasis of breast cancer. (C) 2000 Academic Press.
| Original language | English |
|---|---|
| Pages (from-to) | 60-62 |
| Number of pages | 3 |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 275 |
| Issue number | 1 |
| DOIs | |
| State | Published - Aug 18 2000 |
Fingerprint
Keywords
- Breast cancer
- Interleukin-1α
- Metastasis
- Nuclear factor-κB
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
Cite this
Cancer cell-derived interleukin 1α contributes to autocrine and paracrine induction of pro-metastatic genes in breast cancer. / Nozaki, Shinichi; Sledge, George W.; Nakshatri, Harikrishna.
In: Biochemical and Biophysical Research Communications, Vol. 275, No. 1, 18.08.2000, p. 60-62.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Cancer cell-derived interleukin 1α contributes to autocrine and paracrine induction of pro-metastatic genes in breast cancer
AU - Nozaki, Shinichi
AU - Sledge, George W.
AU - Nakshatri, Harikrishna
PY - 2000/8/18
Y1 - 2000/8/18
N2 - Invasion and metastasis of cancer cells is a complex process requiring the activity of proteins that promote extracellular matrix degradation, motility of cancer cells, and angiogenesis. Although exclusively the cancer cells make several of these proteins, few key proteins are derived from stromal cells in response to cancer cell-stromal cell interaction. In this report, we show that the breast cancer cell-derived interleukin-1alpha (IL-1α) plays an important role in expression of pro-metastatic genes in cancer as well as in stromal cells. Neutralizing antibody against IL-1α inhibited IL-6, and IL-8 expression in IL-1α-expressing cancer cells. In addition, this antibody also prevented induction of IL-6, IL-8, and matrix metalloproteinase 3 (MMP3) but not vascular endothelial growth factor (VEGF) in fibroblasts by conditioned medium (CM) from IL-1α-expressing breast cancer cells. These resuits suggest that inhibition of IL-1α activity by either neutralizing antibody against IL-1α or chemical inhibitor of IL-1α processing may prevent invasion and metastasis of breast cancer. (C) 2000 Academic Press.
AB - Invasion and metastasis of cancer cells is a complex process requiring the activity of proteins that promote extracellular matrix degradation, motility of cancer cells, and angiogenesis. Although exclusively the cancer cells make several of these proteins, few key proteins are derived from stromal cells in response to cancer cell-stromal cell interaction. In this report, we show that the breast cancer cell-derived interleukin-1alpha (IL-1α) plays an important role in expression of pro-metastatic genes in cancer as well as in stromal cells. Neutralizing antibody against IL-1α inhibited IL-6, and IL-8 expression in IL-1α-expressing cancer cells. In addition, this antibody also prevented induction of IL-6, IL-8, and matrix metalloproteinase 3 (MMP3) but not vascular endothelial growth factor (VEGF) in fibroblasts by conditioned medium (CM) from IL-1α-expressing breast cancer cells. These resuits suggest that inhibition of IL-1α activity by either neutralizing antibody against IL-1α or chemical inhibitor of IL-1α processing may prevent invasion and metastasis of breast cancer. (C) 2000 Academic Press.
KW - Breast cancer
KW - Interleukin-1α
KW - Metastasis
KW - Nuclear factor-κB
UR - http://www.scopus.com/inward/record.url?scp=0034682914&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0034682914&partnerID=8YFLogxK
U2 - 10.1006/bbrc.2000.3241
DO - 10.1006/bbrc.2000.3241
M3 - Article
C2 - 10944441
AN - SCOPUS:0034682914
VL - 275
SP - 60
EP - 62
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 1
ER -