Cancer cell-derived interleukin 1α contributes to autocrine and paracrine induction of pro-metastatic genes in breast cancer

Shinichi Nozaki, George W. Sledge, Harikrishna Nakshatri

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

Invasion and metastasis of cancer cells is a complex process requiring the activity of proteins that promote extracellular matrix degradation, motility of cancer cells, and angiogenesis. Although exclusively the cancer cells make several of these proteins, few key proteins are derived from stromal cells in response to cancer cell-stromal cell interaction. In this report, we show that the breast cancer cell-derived interleukin-1alpha (IL-1α) plays an important role in expression of pro-metastatic genes in cancer as well as in stromal cells. Neutralizing antibody against IL-1α inhibited IL-6, and IL-8 expression in IL-1α-expressing cancer cells. In addition, this antibody also prevented induction of IL-6, IL-8, and matrix metalloproteinase 3 (MMP3) but not vascular endothelial growth factor (VEGF) in fibroblasts by conditioned medium (CM) from IL-1α-expressing breast cancer cells. These resuits suggest that inhibition of IL-1α activity by either neutralizing antibody against IL-1α or chemical inhibitor of IL-1α processing may prevent invasion and metastasis of breast cancer. (C) 2000 Academic Press.

Original languageEnglish
Pages (from-to)60-62
Number of pages3
JournalBiochemical and Biophysical Research Communications
Volume275
Issue number1
DOIs
StatePublished - Aug 18 2000

Fingerprint

Interleukin-1alpha
Interleukin-1
Genes
Cells
Breast Neoplasms
Stromal Cells
Neoplasms
Neutralizing Antibodies
Interleukin-8
Interleukin-6
Neoplasm Metastasis
Matrix Metalloproteinase 3
Extracellular Matrix Proteins
Neoplasm Genes
Fibroblasts
Conditioned Culture Medium
Cell Communication
Vascular Endothelial Growth Factor A
Cell Movement
Proteins

Keywords

  • Breast cancer
  • Interleukin-1α
  • Metastasis
  • Nuclear factor-κB

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Cancer cell-derived interleukin 1α contributes to autocrine and paracrine induction of pro-metastatic genes in breast cancer. / Nozaki, Shinichi; Sledge, George W.; Nakshatri, Harikrishna.

In: Biochemical and Biophysical Research Communications, Vol. 275, No. 1, 18.08.2000, p. 60-62.

Research output: Contribution to journalArticle

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