CAP defines a second signalling pathway required for insulin-stimulated glucose transport

Christian A. Baumann, Vered Ribon, Makoto Kanzaki, Debbie C. Thurmond, Silvia Mora, Satoshi Shigematsu, Perry E. Bickel, Jeffrey E. Pessin, Alan R. Saltiel

Research output: Contribution to journalArticle

524 Citations (Scopus)

Abstract

Insulin stimulates the transport of glucose into fat and muscle cells. Although the precise molecular mechanisms involved in this process remain uncertain, insulin initiates its actions by binding to its tyrosine kinase receptor, leading to the phosphorylation of intracellular substrates. One such substrate is the Cbl proto-oncogene product. Cbl is recruited to the insulin receptor by interaction with the adapter protein CAP, through one of three adjacent SH3 domains in the carboxy terminus of CAP. Upon phosphorylation of Cbl, the CAP-Cbl complex dissociates from the insulin receptor and moves to a caveolin-enriched, triton-insoluble membrane fraction. Here, to identify a molecular mechanism underlying this subcellular redistribution, we screened a yeast two-hybrid library using the amino-terminal region of CAP and identified the caveolar protein flotillin. Flotillin forms a ternary complex with CAP and Cbl, directing the localization of the CAP-Cbl complex to a lipid raft subdomain of the plasma membrane. Expression of the N-terminal domain of CAP in 3T3-L1 adipocytes blocks the stimulation of glucose transport by insulin, without affecting signalling events that depend on phosphatidylinositol-3-OH kinase. Thus, localization of the Cbl-CAP complex to lipid rafts generates a pathway that is crucial in the regulation of glucose uptake.

Original languageEnglish (US)
Pages (from-to)202-207
Number of pages6
JournalNature
Volume407
Issue number6801
DOIs
StatePublished - Sep 14 2000
Externally publishedYes

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Insulin Receptor
Insulin
Adipocytes
Glucose
Phosphorylation
Caveolins
Lipids
src Homology Domains
Proto-Oncogenes
Oncogene Proteins
Receptor Protein-Tyrosine Kinases
Phosphatidylinositol 3-Kinases
Muscle Cells
Libraries
Proteins
Yeasts
Cell Membrane
Membranes
flotillins

ASJC Scopus subject areas

  • General

Cite this

Baumann, C. A., Ribon, V., Kanzaki, M., Thurmond, D. C., Mora, S., Shigematsu, S., ... Saltiel, A. R. (2000). CAP defines a second signalling pathway required for insulin-stimulated glucose transport. Nature, 407(6801), 202-207. https://doi.org/10.1038/35025089

CAP defines a second signalling pathway required for insulin-stimulated glucose transport. / Baumann, Christian A.; Ribon, Vered; Kanzaki, Makoto; Thurmond, Debbie C.; Mora, Silvia; Shigematsu, Satoshi; Bickel, Perry E.; Pessin, Jeffrey E.; Saltiel, Alan R.

In: Nature, Vol. 407, No. 6801, 14.09.2000, p. 202-207.

Research output: Contribution to journalArticle

Baumann, CA, Ribon, V, Kanzaki, M, Thurmond, DC, Mora, S, Shigematsu, S, Bickel, PE, Pessin, JE & Saltiel, AR 2000, 'CAP defines a second signalling pathway required for insulin-stimulated glucose transport', Nature, vol. 407, no. 6801, pp. 202-207. https://doi.org/10.1038/35025089
Baumann CA, Ribon V, Kanzaki M, Thurmond DC, Mora S, Shigematsu S et al. CAP defines a second signalling pathway required for insulin-stimulated glucose transport. Nature. 2000 Sep 14;407(6801):202-207. https://doi.org/10.1038/35025089
Baumann, Christian A. ; Ribon, Vered ; Kanzaki, Makoto ; Thurmond, Debbie C. ; Mora, Silvia ; Shigematsu, Satoshi ; Bickel, Perry E. ; Pessin, Jeffrey E. ; Saltiel, Alan R. / CAP defines a second signalling pathway required for insulin-stimulated glucose transport. In: Nature. 2000 ; Vol. 407, No. 6801. pp. 202-207.
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