Carboxyatractylate-sensitive uncoupling in liver mitochondria from ground squirrels during hibernation and arousal

N. N. Brustovetsky, Z. G. Amerkanov, M. E. Yegorova, E. N. Mokhova, V. P. Skulachev

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Energy coupling parameters of liver mitochondria from hibernating and arousing ground squirrels have been studied. In the oligomycin-treated mitochondria, carboxyatractylate, an inhibitor of the ATP/ADP-antiporter, is shown to decrease the respiration rate, to increase the membrane potential and to lower the rate of the membrane-potential discharge after the addition of cyanide to liver mitochondria from hibernating and arousing animals. BSA effectively substitutes for carboxyactactylate so that carboxyactactylate, added after BSA, has no effect. In mitochondria from hibernating animals, the maximal respiration rate in the presence of DNP and the rate of the membrane potential discharge in its absence are much lower than in those from arousing animals. It has been concluded that upon arousal of the animals from hibernation, the uncouling of oxidative phosphorylation, mediated by free fatty acids and ATP/ADP-antiporter, parallels the respiratory chain activation.

Original languageEnglish (US)
Pages (from-to)190-192
Number of pages3
JournalFEBS Letters
Volume272
Issue number1-2
DOIs
StatePublished - Oct 15 1990

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Hibernation
Sciuridae
Mitochondria
Liver Mitochondrion
Arousal
Liver
Animals
Membrane Potentials
Antiporters
Respiratory Rate
Membranes
Adenosine Diphosphate
Adenosine Triphosphate
Oligomycins
Oxidative Phosphorylation
Cyanides
Electron Transport
Nonesterified Fatty Acids
Chemical activation
carboxyatractyloside

Keywords

  • ATP/ADP-antiporter
  • Fatty acid
  • Hibernation
  • Uncoupling

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Carboxyatractylate-sensitive uncoupling in liver mitochondria from ground squirrels during hibernation and arousal. / Brustovetsky, N. N.; Amerkanov, Z. G.; Yegorova, M. E.; Mokhova, E. N.; Skulachev, V. P.

In: FEBS Letters, Vol. 272, No. 1-2, 15.10.1990, p. 190-192.

Research output: Contribution to journalArticle

Brustovetsky, N. N. ; Amerkanov, Z. G. ; Yegorova, M. E. ; Mokhova, E. N. ; Skulachev, V. P. / Carboxyatractylate-sensitive uncoupling in liver mitochondria from ground squirrels during hibernation and arousal. In: FEBS Letters. 1990 ; Vol. 272, No. 1-2. pp. 190-192.
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