Carboxyatractylate-sensitive uncoupling in liver mitochondria from ground squirrels during hibernation and arousal

N. N. Brustovetsky, Z. G. Amerkanov, M. E. Yegorova, E. N. Mokhova, V. P. Skulachev

Research output: Contribution to journalArticlepeer-review

30 Scopus citations

Abstract

Energy coupling parameters of liver mitochondria from hibernating and arousing ground squirrels have been studied. In the oligomycin-treated mitochondria, carboxyatractylate, an inhibitor of the ATP/ADP-antiporter, is shown to decrease the respiration rate, to increase the membrane potential and to lower the rate of the membrane-potential discharge after the addition of cyanide to liver mitochondria from hibernating and arousing animals. BSA effectively substitutes for carboxyactactylate so that carboxyactactylate, added after BSA, has no effect. In mitochondria from hibernating animals, the maximal respiration rate in the presence of DNP and the rate of the membrane potential discharge in its absence are much lower than in those from arousing animals. It has been concluded that upon arousal of the animals from hibernation, the uncouling of oxidative phosphorylation, mediated by free fatty acids and ATP/ADP-antiporter, parallels the respiratory chain activation.

Original languageEnglish (US)
Pages (from-to)190-192
Number of pages3
JournalFEBS Letters
Volume272
Issue number1-2
DOIs
StatePublished - Oct 15 1990
Externally publishedYes

Keywords

  • ATP/ADP-antiporter
  • Fatty acid
  • Hibernation
  • Uncoupling

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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