Cardiac defects, nuchal edema and abnormal lymphatic development are not associated with morphological changes in the ductus venosus

Nicole B. Burger, Monique C. Haak, Evelien Kok, Christianne J M de Groot, Weinian Shou, Peter J. Scambler, Youngsook Lee, Eunjin Cho, Vincent M. Christoffels, Mireille N. Bekker

Research output: Contribution to journalArticle

Abstract

Background In human fetuses with cardiac defects and increased nuchal translucency, abnormal ductus venosus flow velocity waveforms are observed. It is unknown whether abnormal ductus venosus flow velocity waveforms in fetuses with increased nuchal translucency are a reflection of altered cardiac function or are caused by local morphological alterations in the ductus venosus. Aim The aim of this study was to investigate if the observed increased nuchal translucency, cardiac defects and abnormal lymphatic development in the examined mouse models are associated with local changes in ductus venosus morphology. Study design Mouse embryos with anomalous lymphatic development and nuchal edema (Ccbe1−/− embryos), mouse embryos with cardiac defects and nuchal edema (Fkbp12−/−, Tbx1−/−, Chd7fl/fl;Mesp1Cre, Jarid2−/− NE+ embryos) and mouse embryos with cardiac defects without nuchal edema (Tbx2−/−, Fgf10−/−, Jarid2−/− NE − embryos) were examined. Embryos were analyzed from embryonic day (E) 11.5 to 15.5 using markers for endothelium, smooth muscle actin, nerve tissue and elastic fibers. Results All mutant and wild-type mouse embryos showed similar, positive endothelial and smooth muscle cell expression in the ductus venosus at E11.5–15.5. Nerve marker and elastic fiber expression were not identified in the ductus venosus in all investigated mutant and wild-type embryos. Local morphology and expression of the used markers were similar in the ductus venosus in all examined mutant and wild-type embryos. Conclusions Cardiac defects, nuchal edema and abnormal lymphatic development are not associated with morphological changes in the ductus venosus. Ductus venosus flow velocity waveforms most probably reflect intracardiac pressure.

Original languageEnglish (US)
Pages (from-to)39-48
Number of pages10
JournalEarly Human Development
Volume101
DOIs
StatePublished - Oct 1 2016

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Edema
Embryonic Structures
Nuchal Translucency Measurement
Elastic Tissue
Nerve Fibers
Fetus
Nerve Tissue
Smooth Muscle Myocytes
Endothelium
Smooth Muscle
Actins
Pressure

Keywords

  • Cardiac defect
  • Ductus venosus
  • Endothelium
  • Increased nuchal translucency
  • Morphology
  • Nuchal edema

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health
  • Obstetrics and Gynecology

Cite this

Cardiac defects, nuchal edema and abnormal lymphatic development are not associated with morphological changes in the ductus venosus. / Burger, Nicole B.; Haak, Monique C.; Kok, Evelien; de Groot, Christianne J M; Shou, Weinian; Scambler, Peter J.; Lee, Youngsook; Cho, Eunjin; Christoffels, Vincent M.; Bekker, Mireille N.

In: Early Human Development, Vol. 101, 01.10.2016, p. 39-48.

Research output: Contribution to journalArticle

Burger, Nicole B. ; Haak, Monique C. ; Kok, Evelien ; de Groot, Christianne J M ; Shou, Weinian ; Scambler, Peter J. ; Lee, Youngsook ; Cho, Eunjin ; Christoffels, Vincent M. ; Bekker, Mireille N. / Cardiac defects, nuchal edema and abnormal lymphatic development are not associated with morphological changes in the ductus venosus. In: Early Human Development. 2016 ; Vol. 101. pp. 39-48.
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title = "Cardiac defects, nuchal edema and abnormal lymphatic development are not associated with morphological changes in the ductus venosus",
abstract = "Background In human fetuses with cardiac defects and increased nuchal translucency, abnormal ductus venosus flow velocity waveforms are observed. It is unknown whether abnormal ductus venosus flow velocity waveforms in fetuses with increased nuchal translucency are a reflection of altered cardiac function or are caused by local morphological alterations in the ductus venosus. Aim The aim of this study was to investigate if the observed increased nuchal translucency, cardiac defects and abnormal lymphatic development in the examined mouse models are associated with local changes in ductus venosus morphology. Study design Mouse embryos with anomalous lymphatic development and nuchal edema (Ccbe1−/− embryos), mouse embryos with cardiac defects and nuchal edema (Fkbp12−/−, Tbx1−/−, Chd7fl/fl;Mesp1Cre, Jarid2−/− NE+ embryos) and mouse embryos with cardiac defects without nuchal edema (Tbx2−/−, Fgf10−/−, Jarid2−/− NE − embryos) were examined. Embryos were analyzed from embryonic day (E) 11.5 to 15.5 using markers for endothelium, smooth muscle actin, nerve tissue and elastic fibers. Results All mutant and wild-type mouse embryos showed similar, positive endothelial and smooth muscle cell expression in the ductus venosus at E11.5–15.5. Nerve marker and elastic fiber expression were not identified in the ductus venosus in all investigated mutant and wild-type embryos. Local morphology and expression of the used markers were similar in the ductus venosus in all examined mutant and wild-type embryos. Conclusions Cardiac defects, nuchal edema and abnormal lymphatic development are not associated with morphological changes in the ductus venosus. Ductus venosus flow velocity waveforms most probably reflect intracardiac pressure.",
keywords = "Cardiac defect, Ductus venosus, Endothelium, Increased nuchal translucency, Morphology, Nuchal edema",
author = "Burger, {Nicole B.} and Haak, {Monique C.} and Evelien Kok and {de Groot}, {Christianne J M} and Weinian Shou and Scambler, {Peter J.} and Youngsook Lee and Eunjin Cho and Christoffels, {Vincent M.} and Bekker, {Mireille N.}",
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T1 - Cardiac defects, nuchal edema and abnormal lymphatic development are not associated with morphological changes in the ductus venosus

AU - Burger, Nicole B.

AU - Haak, Monique C.

AU - Kok, Evelien

AU - de Groot, Christianne J M

AU - Shou, Weinian

AU - Scambler, Peter J.

AU - Lee, Youngsook

AU - Cho, Eunjin

AU - Christoffels, Vincent M.

AU - Bekker, Mireille N.

PY - 2016/10/1

Y1 - 2016/10/1

N2 - Background In human fetuses with cardiac defects and increased nuchal translucency, abnormal ductus venosus flow velocity waveforms are observed. It is unknown whether abnormal ductus venosus flow velocity waveforms in fetuses with increased nuchal translucency are a reflection of altered cardiac function or are caused by local morphological alterations in the ductus venosus. Aim The aim of this study was to investigate if the observed increased nuchal translucency, cardiac defects and abnormal lymphatic development in the examined mouse models are associated with local changes in ductus venosus morphology. Study design Mouse embryos with anomalous lymphatic development and nuchal edema (Ccbe1−/− embryos), mouse embryos with cardiac defects and nuchal edema (Fkbp12−/−, Tbx1−/−, Chd7fl/fl;Mesp1Cre, Jarid2−/− NE+ embryos) and mouse embryos with cardiac defects without nuchal edema (Tbx2−/−, Fgf10−/−, Jarid2−/− NE − embryos) were examined. Embryos were analyzed from embryonic day (E) 11.5 to 15.5 using markers for endothelium, smooth muscle actin, nerve tissue and elastic fibers. Results All mutant and wild-type mouse embryos showed similar, positive endothelial and smooth muscle cell expression in the ductus venosus at E11.5–15.5. Nerve marker and elastic fiber expression were not identified in the ductus venosus in all investigated mutant and wild-type embryos. Local morphology and expression of the used markers were similar in the ductus venosus in all examined mutant and wild-type embryos. Conclusions Cardiac defects, nuchal edema and abnormal lymphatic development are not associated with morphological changes in the ductus venosus. Ductus venosus flow velocity waveforms most probably reflect intracardiac pressure.

AB - Background In human fetuses with cardiac defects and increased nuchal translucency, abnormal ductus venosus flow velocity waveforms are observed. It is unknown whether abnormal ductus venosus flow velocity waveforms in fetuses with increased nuchal translucency are a reflection of altered cardiac function or are caused by local morphological alterations in the ductus venosus. Aim The aim of this study was to investigate if the observed increased nuchal translucency, cardiac defects and abnormal lymphatic development in the examined mouse models are associated with local changes in ductus venosus morphology. Study design Mouse embryos with anomalous lymphatic development and nuchal edema (Ccbe1−/− embryos), mouse embryos with cardiac defects and nuchal edema (Fkbp12−/−, Tbx1−/−, Chd7fl/fl;Mesp1Cre, Jarid2−/− NE+ embryos) and mouse embryos with cardiac defects without nuchal edema (Tbx2−/−, Fgf10−/−, Jarid2−/− NE − embryos) were examined. Embryos were analyzed from embryonic day (E) 11.5 to 15.5 using markers for endothelium, smooth muscle actin, nerve tissue and elastic fibers. Results All mutant and wild-type mouse embryos showed similar, positive endothelial and smooth muscle cell expression in the ductus venosus at E11.5–15.5. Nerve marker and elastic fiber expression were not identified in the ductus venosus in all investigated mutant and wild-type embryos. Local morphology and expression of the used markers were similar in the ductus venosus in all examined mutant and wild-type embryos. Conclusions Cardiac defects, nuchal edema and abnormal lymphatic development are not associated with morphological changes in the ductus venosus. Ductus venosus flow velocity waveforms most probably reflect intracardiac pressure.

KW - Cardiac defect

KW - Ductus venosus

KW - Endothelium

KW - Increased nuchal translucency

KW - Morphology

KW - Nuchal edema

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