Cardiac engraftment of genetically-selected parthenogenetic stem cell-derived cardiomyocytes

Tao Yang, Michael Rubart-von der Lohe, Mark Soonpaa, Michael Didié, Peter Christalla, Wolfram Hubertus Zimmermann, Loren Field

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Parthenogenetic stem cells (PSCs) are a promising candidate donor for cell therapy applications. Similar to embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs), PSCs exhibit self-renewing capacity and clonogenic proliferation in vitro. PSCs exhibit largely haploidentical genotype, and as such may constitute an attractive population for allogenic applications. In this study, PSCs isolated from transgenic mice carrying a cardiomyocyte-restricted reporter transgene to permit tracking of donor cells were genetically modified to carry a cardiomyocyte-restricted aminoglycoside phosphotransferase expression cassette (MHC-neo<sup>r</sup>/pGK-hygro<sup>r</sup>) to permit the generation of highly enriched cardiomyocyte cultures from spontaneously differentiating PSCs by simple selection with the neomycin analogue G148. Following engraftment into isogenic recipient hearts, the selected cardiomyocytes formed a functional syncytium with the host myocardium as evidenced by the presence of entrained intracellular calcium transients. These cells thus constitute a potential source of therapeutic donor cells.

Original languageEnglish
Article numbere0131511
JournalPLoS One
Volume10
Issue number6
DOIs
StatePublished - Jun 25 2015

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Stem cells
Cardiac Myocytes
stem cells
Stem Cells
Kanamycin Kinase
Cell Tracking
cells
Induced Pluripotent Stem Cells
aminoglycosides
therapeutics
Neomycin
neomycin
embryonic stem cells
giant cells
Giant Cells
Embryonic Stem Cells
Cell- and Tissue-Based Therapy
myocardium
Transgenes
Transgenic Mice

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Cardiac engraftment of genetically-selected parthenogenetic stem cell-derived cardiomyocytes. / Yang, Tao; Rubart-von der Lohe, Michael; Soonpaa, Mark; Didié, Michael; Christalla, Peter; Zimmermann, Wolfram Hubertus; Field, Loren.

In: PLoS One, Vol. 10, No. 6, e0131511, 25.06.2015.

Research output: Contribution to journalArticle

Yang, Tao ; Rubart-von der Lohe, Michael ; Soonpaa, Mark ; Didié, Michael ; Christalla, Peter ; Zimmermann, Wolfram Hubertus ; Field, Loren. / Cardiac engraftment of genetically-selected parthenogenetic stem cell-derived cardiomyocytes. In: PLoS One. 2015 ; Vol. 10, No. 6.
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