Cardiac engraftment of genetically-selected parthenogenetic stem cell-derived cardiomyocytes

Tao Yang, Michael Rubart, Mark H. Soonpaa, Michael Didié, Peter Christalla, Wolfram Hubertus Zimmermann, Loren J. Field

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

Parthenogenetic stem cells (PSCs) are a promising candidate donor for cell therapy applications. Similar to embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs), PSCs exhibit self-renewing capacity and clonogenic proliferation in vitro. PSCs exhibit largely haploidentical genotype, and as such may constitute an attractive population for allogenic applications. In this study, PSCs isolated from transgenic mice carrying a cardiomyocyte-restricted reporter transgene to permit tracking of donor cells were genetically modified to carry a cardiomyocyte-restricted aminoglycoside phosphotransferase expression cassette (MHC-neo<sup>r</sup>/pGK-hygro<sup>r</sup>) to permit the generation of highly enriched cardiomyocyte cultures from spontaneously differentiating PSCs by simple selection with the neomycin analogue G148. Following engraftment into isogenic recipient hearts, the selected cardiomyocytes formed a functional syncytium with the host myocardium as evidenced by the presence of entrained intracellular calcium transients. These cells thus constitute a potential source of therapeutic donor cells.

Original languageEnglish (US)
Article numbere0131511
JournalPLoS ONE
Volume10
Issue number6
DOIs
StatePublished - Jun 25 2015

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

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