Cardiac UCP2 expression and myocardial oxidative metabolism during acute septic shock in the rat.

Michael J. Roshon, Jeffrey Kline, Lisa R. Thornton, John A. Watts

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Septic shock decreases cardiac hydraulic work relative to the rate of myocardial oxygen consumption, causing decreased mechanical efficiency (hydraulic work/myocardial oxygen consumption). This study tested whether the mitochondrial uncoupling protein UCP2 was responsible for decreased cardiac mechanical efficiency after polymicrobial septic shock. Sepsis was initiated in ketamine/xylazine-anesthetized rats by cecal ligation and puncture (CLP). Steady-state mRNA content was quantified by Northern blot analysis, and protein content was estimated by western blot. Additional hearts were removed after 12 h and perfused in working mode to measure work (mmHg x mL/min/100 g dry wt) and efficiency (CE = work/oxygen consumption, %). The 72-h mortality rate was 80%, and deaths occurred between 12-32 h. Cardiac work (152 +/- 15, shock vs. 235 +/- 16, control; P <0.05) and cardiac efficiency (4.0 +/- 0.4 vs. 5.6 +/- 0.3; P <0.05) were significantly decreased when hearts were isolated 12 h after CLP. Myocardial UCP2 mRNA expression was increased by 52% (12 h) compared with control hearts; however, there was no detectable UCP2 protein in mitochondria isolated from either control or septic hearts. Conclusions: Although polymicrobial sepsis decreased cardiac mechanical efficiency and increased UCP-2 expression coincident with premortal hypothermia, we did not detect any evidence of UCP-2 protein in septic heart muscle. These data argue against the hypothesis that UCP-2 causes decreased cardiac mechanical efficiency in septic shock.

Original languageEnglish (US)
Pages (from-to)570-576
Number of pages7
JournalShock
Volume19
Issue number6
StatePublished - Jun 2003
Externally publishedYes

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Septic Shock
Oxygen Consumption
Punctures
Ligation
Sepsis
Xylazine
Messenger RNA
Ketamine
Hypothermia
Northern Blotting
Shock
Myocardium
Mitochondria
Proteins
Western Blotting
Mortality

ASJC Scopus subject areas

  • Critical Care and Intensive Care Medicine
  • Physiology

Cite this

Cardiac UCP2 expression and myocardial oxidative metabolism during acute septic shock in the rat. / Roshon, Michael J.; Kline, Jeffrey; Thornton, Lisa R.; Watts, John A.

In: Shock, Vol. 19, No. 6, 06.2003, p. 570-576.

Research output: Contribution to journalArticle

Roshon, MJ, Kline, J, Thornton, LR & Watts, JA 2003, 'Cardiac UCP2 expression and myocardial oxidative metabolism during acute septic shock in the rat.', Shock, vol. 19, no. 6, pp. 570-576.
Roshon, Michael J. ; Kline, Jeffrey ; Thornton, Lisa R. ; Watts, John A. / Cardiac UCP2 expression and myocardial oxidative metabolism during acute septic shock in the rat. In: Shock. 2003 ; Vol. 19, No. 6. pp. 570-576.
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