Cell death can occur through various mechanisms. In this study, we investigated whether apoptotic mechanisms were active in myocardial cell death in a canine model of myocardial ischemia / reperfusion (Hahn et al, 1992 JPET 260:3). Animals subjected to one hour of ischemia and five hours of reperfusion demonstrated evidence of apoptotic cell death utilizing in situ enzymatic labeling of DNA in 44 +/- 2 % of myocytes in zones of infarct. Animals subjected to a six hour ischémie insult without reperfusion demonstrated 12 +/- 4% labeling whereas normal dog myocardium had less than 1% detectable apoptosis. Parallel agarose gel electrophoresis could demonstrate DNA ladder patterns in the tissue samples of the ischemic/reperfused infarct zones indicative of DNA fragmentation. Corresponding transmission electron microscopy of all experimental groups will be discussed for morphological correlations of cell death. The results suggest that reperfusion contributes significantly to cardiomyocyte apoptosis in this model and that these techniques may be useful for measuring effects of various agents on reperfusion injury.
|Original language||English (US)|
|State||Published - Dec 1 1996|
ASJC Scopus subject areas
- Molecular Biology