Cardiovascular effects of homologous bradykinin in rainbow trout

Kenneth Olson, Daniel J. Conklin, Leroy Weaver, Douglas W. Duff, Ceil A. Herman, Xinying Wang, J. Michael Conlon

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Bradykinins have only recently been identified in fish, and a detailed analysis of their cardiovascular actions is lacking. The present study examines the cardiovascular effects of trout bradykinin ([Arg 0,Trp 6,Leu 8]bradykinin; tBK) in conscious trout, Oncorhynchus mykiss. tBK (1-10 nmol/kg body wt bolus) produced triphasic pressor-depressor-pressor responses. In phase 1, cardiac output (CO), ventral aortic (PVA), dorsal aortic (PDA), and central venous pressure increased, whereas systemic (AS) and gill resistance CRo) were unchanged. In phase 2, RQ increased, whereas R$, CO, and heart rate decreased, reducing PVA and PDA- Plasma prostaglandin E 2 and the prostacyclin metabolite, 6-ketoprostaglandin Fla, were significantly elevated during phase 2, whereas leukotrienes C 4 and B 4 and thromboxane B 2 were unaffected. Phase 3 was produced by an increased CO and AS and the return of äG to control. Phase 1 presser response was not blocked by inhibitors of cyclooxygenase, angiotensin-converting enzyme (ACE) or α-adrenoceptors (a-AD), whereas phase 2 depressor and plasma prostaglandin responses were prevented by cyclooxygenase inhibition. Phase 3 was partially blocked by ACE and α-AD inhibitors and is a response to the preceding hypotension. In vitro, tBK only decreased vascular resistance in the perfused splanchnic or skeletal muscle-kidney preparations. These results show that although tBK has multiple effects on the trout cardiovascular system, none of the effects are due to direct tBK stimulation of vascular smooth muscle. Phase 2 vasodilation has features consistent with release of vasodilator prostaglandins while the mechanism of phase 1 constriction is unknown.

Original languageEnglish
JournalThe American journal of physiology
Volume272
Issue number4 PART 2
StatePublished - 1997

Fingerprint

Trout
Oncorhynchus mykiss
Bradykinin
Cardiac Output
Angiotensin-Converting Enzyme Inhibitors
Prostaglandins
Leukotriene C4
Central Venous Pressure
Leukotriene B4
Viscera
Thromboxanes
Epoprostenol
Prostaglandin-Endoperoxide Synthases
Cardiovascular System
Prostaglandins E
Vasodilator Agents
Vascular Smooth Muscle
Constriction
Vasodilation
Vascular Resistance

Keywords

  • Endothelium-derived relaxing factor
  • Kallikrein-kinin system
  • Prostaglandins

ASJC Scopus subject areas

  • Physiology (medical)

Cite this

Olson, K., Conklin, D. J., Weaver, L., Duff, D. W., Herman, C. A., Wang, X., & Michael Conlon, J. (1997). Cardiovascular effects of homologous bradykinin in rainbow trout. The American journal of physiology, 272(4 PART 2).

Cardiovascular effects of homologous bradykinin in rainbow trout. / Olson, Kenneth; Conklin, Daniel J.; Weaver, Leroy; Duff, Douglas W.; Herman, Ceil A.; Wang, Xinying; Michael Conlon, J.

In: The American journal of physiology, Vol. 272, No. 4 PART 2, 1997.

Research output: Contribution to journalArticle

Olson, K, Conklin, DJ, Weaver, L, Duff, DW, Herman, CA, Wang, X & Michael Conlon, J 1997, 'Cardiovascular effects of homologous bradykinin in rainbow trout', The American journal of physiology, vol. 272, no. 4 PART 2.
Olson K, Conklin DJ, Weaver L, Duff DW, Herman CA, Wang X et al. Cardiovascular effects of homologous bradykinin in rainbow trout. The American journal of physiology. 1997;272(4 PART 2).
Olson, Kenneth ; Conklin, Daniel J. ; Weaver, Leroy ; Duff, Douglas W. ; Herman, Ceil A. ; Wang, Xinying ; Michael Conlon, J. / Cardiovascular effects of homologous bradykinin in rainbow trout. In: The American journal of physiology. 1997 ; Vol. 272, No. 4 PART 2.
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