Caries progression and inhibition in human and bovine root dentine in situ

Anderson Hara, C. S. Queiroz, A. F. Paes Leme, M. C. Serra, Jaime A. Cury

Research output: Contribution to journalArticle

81 Citations (Scopus)

Abstract

Since the use of bovine instead of human dentine to evaluate cariogenic and anticariogenic substances is not well established, this in situ study was conducted. Eleven volunteers wore palatal acrylic devices containing 4 dentine slabs (2 human and 2 bovine). Sucrose solution (20%) was dripped over all slabs 4 times a day, simulating a cariogenic challenge. Dentifrice slurries, fluoridated or not, were dripped over specified dentine slabs 3 times a day to evaluate caries reduction. After 14 days, the biofilm formed on the dentine slabs was collected for microbiological analysis. In dentine, mineral loss (ΔZ) and lesion depth (LD) were determined by cross-sectional microhardness and by polarized light microscopy, respectively. The total streptococci and mutans streptococci counts in the biofilm formed either on human or on bovine slabs, whether treated or not with fluoride dentifrice, were not statistically different. The ΔZ and the LD values of dentine treated with fluoride dentifrice were significantly lower than the values of dentine treated with non-fluoride dentifrice. The differences in the ΔZ and LD values between the human and bovine dentine were not statistically significant. The results suggest that bovine dentine can be used instead of human to evaluate caries development and inhibition.

Original languageEnglish (US)
Pages (from-to)339-344
Number of pages6
JournalCaries Research
Volume37
Issue number5
DOIs
StatePublished - Sep 2003
Externally publishedYes

Fingerprint

Dentin
Dentifrices
Biofilms
Fluorides
Polarization Microscopy
Streptococcus mutans
Streptococcus
Minerals
Sucrose
Volunteers
Equipment and Supplies

Keywords

  • Bovine dentine
  • Dentifrice
  • Fluoride
  • Human dentine
  • Root caries

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Hara, A., Queiroz, C. S., Paes Leme, A. F., Serra, M. C., & Cury, J. A. (2003). Caries progression and inhibition in human and bovine root dentine in situ. Caries Research, 37(5), 339-344. https://doi.org/10.1159/000072165

Caries progression and inhibition in human and bovine root dentine in situ. / Hara, Anderson; Queiroz, C. S.; Paes Leme, A. F.; Serra, M. C.; Cury, Jaime A.

In: Caries Research, Vol. 37, No. 5, 09.2003, p. 339-344.

Research output: Contribution to journalArticle

Hara, A, Queiroz, CS, Paes Leme, AF, Serra, MC & Cury, JA 2003, 'Caries progression and inhibition in human and bovine root dentine in situ', Caries Research, vol. 37, no. 5, pp. 339-344. https://doi.org/10.1159/000072165
Hara, Anderson ; Queiroz, C. S. ; Paes Leme, A. F. ; Serra, M. C. ; Cury, Jaime A. / Caries progression and inhibition in human and bovine root dentine in situ. In: Caries Research. 2003 ; Vol. 37, No. 5. pp. 339-344.
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