Casein Kinase I Associates with Members of the Centaurin-α Family of Phosphatidylinositol 3,4,5-Trisphosphate-binding Proteins

Thierry Dubois, Preeti Kerai, Eva Zemlickova, Steven Howell, Trevor R. Jackson, Kanamarlapudi Venkateswarlu, Peter J. Cullen, Anne B. Theibert, Louise Larose, Peter Roach, Alastair Aitken

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

Mammalian casein kinases I (CKI) belong to a family of serine/threonine protein kinases involved in diverse cellular processes including cell cycle progression, membrane trafficking, circadian rhythms, and Wnt signaling. Here we show that CKIα co-purifies with centaurin-α1 in brain and that they interact in vitro and form a complex in cells. In addition, we show that the association is direct and occurs through the kinase domain of CKI within a loop comprising residues 217-233. These residues are well conserved in all members of the CKI family, and we show that centaurin-α1 associates in vitro with all mammalian CKI isoforms. To date, CKIα represents the first protein partner identified for centaurin-α 1. However, our data suggest that centaurin-α1 is not a substrate for CKIα and has no effect on CKIα activity. Centaurin-α1 has been identified as a phosphatidylinositol 3,4,5-trisphosphate-binding protein. Centaurin-α1 contains a cysteine-rich domain that is shared by members of a newly identified family of ADP-ribosylation factor guanosine trisphosphatase-activating proteins. These proteins are involved in membrane trafficking and actin cytoskeleton rearrangement, thus supporting a role for CKIα in these biological events.

Original languageEnglish
Pages (from-to)18757-18764
Number of pages8
JournalJournal of Biological Chemistry
Volume276
Issue number22
DOIs
StatePublished - Jun 1 2001

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Casein Kinase I
Carrier Proteins
ADP-Ribosylation Factors
Membranes
phosphatidylinositol 3,4,5-triphosphate
Proteins
Guanosine
Protein-Serine-Threonine Kinases
Circadian Rhythm
Actin Cytoskeleton
Cysteine
Actins
Brain
Cell Cycle
Protein Isoforms
Phosphotransferases
Cells

ASJC Scopus subject areas

  • Biochemistry

Cite this

Dubois, T., Kerai, P., Zemlickova, E., Howell, S., Jackson, T. R., Venkateswarlu, K., ... Aitken, A. (2001). Casein Kinase I Associates with Members of the Centaurin-α Family of Phosphatidylinositol 3,4,5-Trisphosphate-binding Proteins. Journal of Biological Chemistry, 276(22), 18757-18764. https://doi.org/10.1074/jbc.M010005200

Casein Kinase I Associates with Members of the Centaurin-α Family of Phosphatidylinositol 3,4,5-Trisphosphate-binding Proteins. / Dubois, Thierry; Kerai, Preeti; Zemlickova, Eva; Howell, Steven; Jackson, Trevor R.; Venkateswarlu, Kanamarlapudi; Cullen, Peter J.; Theibert, Anne B.; Larose, Louise; Roach, Peter; Aitken, Alastair.

In: Journal of Biological Chemistry, Vol. 276, No. 22, 01.06.2001, p. 18757-18764.

Research output: Contribution to journalArticle

Dubois, T, Kerai, P, Zemlickova, E, Howell, S, Jackson, TR, Venkateswarlu, K, Cullen, PJ, Theibert, AB, Larose, L, Roach, P & Aitken, A 2001, 'Casein Kinase I Associates with Members of the Centaurin-α Family of Phosphatidylinositol 3,4,5-Trisphosphate-binding Proteins', Journal of Biological Chemistry, vol. 276, no. 22, pp. 18757-18764. https://doi.org/10.1074/jbc.M010005200
Dubois, Thierry ; Kerai, Preeti ; Zemlickova, Eva ; Howell, Steven ; Jackson, Trevor R. ; Venkateswarlu, Kanamarlapudi ; Cullen, Peter J. ; Theibert, Anne B. ; Larose, Louise ; Roach, Peter ; Aitken, Alastair. / Casein Kinase I Associates with Members of the Centaurin-α Family of Phosphatidylinositol 3,4,5-Trisphosphate-binding Proteins. In: Journal of Biological Chemistry. 2001 ; Vol. 276, No. 22. pp. 18757-18764.
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