Casein Kinase I Associates with Members of the Centaurin-α Family of Phosphatidylinositol 3,4,5-Trisphosphate-binding Proteins

Thierry Dubois, Preeti Kerai, Eva Zemlickova, Steven Howell, Trevor R. Jackson, Kanamarlapudi Venkateswarlu, Peter J. Cullen, Anne B. Theibert, Louise Larose, Peter J. Roach, Alastair Aitken

Research output: Contribution to journalArticle

47 Scopus citations

Abstract

Mammalian casein kinases I (CKI) belong to a family of serine/threonine protein kinases involved in diverse cellular processes including cell cycle progression, membrane trafficking, circadian rhythms, and Wnt signaling. Here we show that CKIα co-purifies with centaurin-α1 in brain and that they interact in vitro and form a complex in cells. In addition, we show that the association is direct and occurs through the kinase domain of CKI within a loop comprising residues 217-233. These residues are well conserved in all members of the CKI family, and we show that centaurin-α1 associates in vitro with all mammalian CKI isoforms. To date, CKIα represents the first protein partner identified for centaurin-α 1. However, our data suggest that centaurin-α1 is not a substrate for CKIα and has no effect on CKIα activity. Centaurin-α1 has been identified as a phosphatidylinositol 3,4,5-trisphosphate-binding protein. Centaurin-α1 contains a cysteine-rich domain that is shared by members of a newly identified family of ADP-ribosylation factor guanosine trisphosphatase-activating proteins. These proteins are involved in membrane trafficking and actin cytoskeleton rearrangement, thus supporting a role for CKIα in these biological events.

Original languageEnglish (US)
Pages (from-to)18757-18764
Number of pages8
JournalJournal of Biological Chemistry
Volume276
Issue number22
DOIs
StatePublished - Jun 1 2001

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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    Dubois, T., Kerai, P., Zemlickova, E., Howell, S., Jackson, T. R., Venkateswarlu, K., Cullen, P. J., Theibert, A. B., Larose, L., Roach, P. J., & Aitken, A. (2001). Casein Kinase I Associates with Members of the Centaurin-α Family of Phosphatidylinositol 3,4,5-Trisphosphate-binding Proteins. Journal of Biological Chemistry, 276(22), 18757-18764. https://doi.org/10.1074/jbc.M010005200