Catabolism of isobutyrate by colonocytes

Jerzy Jaskiewicz, Yu Zhao, John W. Hawes, Y. Shimomura, David Crabb, Robert Harris

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Isolated colonocytes have more capacity for the oxidation of isobutyrate and α-ketoisovalerate than isolated enterocytes. Both enterocytes and colonocytes express high levels of 3-hydroxyisobutyryl-CoA hydrolase, an enzyme activity important in maintaining low intracellular concentrations of methacrylyl-CoA, a common, potentially toxic intermediate in the catabolic pathways of these compounds. In spite of comparable 3-hydroxyisobutyryl-CoA hydrolase activities in both cell types, and much greater amounts of 3- hydroxyisobutyrate dehydrogenase in colonocytes than in enterocytes, only the colonocytes produced 3-hydroxyisobutyrate as an endproduct of α- ketoisovalerate and isobutyrate catabolism. Butyrate very effectively inhibits isobutyrate catabolism by colonocytes, most likely by competitively inhibiting activation of isobutyrate to its CoA ester. Oleate also inhibits isobutyrate catabolism, but at a site more distal than butyrate. Starvation of rats for 72 h decreased the capacity of colonocytes for butyrate but not isobutyrate catabolism. We conclude that isobutyrate could function as a carbon source for energy and anapleurosis in colonocytes under conditions of defective butyrate oxidation or low butyrate availability.

Original languageEnglish
Pages (from-to)265-270
Number of pages6
JournalArchives of Biochemistry and Biophysics
Volume327
Issue number2
DOIs
StatePublished - Mar 15 1996

Fingerprint

Isobutyrates
Butyrates
3-hydroxyisobutyryl-CoA hydrolase
Enterocytes
3-hydroxyisobutyrate dehydrogenase
Oxidation
Poisons
Enzyme activity
Coenzyme A
Oleic Acid
Starvation
Rats
Esters
Carbon
Chemical activation
Availability
Enzymes

Keywords

  • 3-hydroxyisobutyrate
  • 3-hydroxyisobutyryl-CoA hydrolase
  • branched-chain α-ketoacid dehydrogenase
  • butyrate
  • colon
  • colonocytes
  • enterocytes
  • glucose
  • isobutyrate
  • ketone bodies
  • starvation
  • valine

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Catabolism of isobutyrate by colonocytes. / Jaskiewicz, Jerzy; Zhao, Yu; Hawes, John W.; Shimomura, Y.; Crabb, David; Harris, Robert.

In: Archives of Biochemistry and Biophysics, Vol. 327, No. 2, 15.03.1996, p. 265-270.

Research output: Contribution to journalArticle

Jaskiewicz, Jerzy ; Zhao, Yu ; Hawes, John W. ; Shimomura, Y. ; Crabb, David ; Harris, Robert. / Catabolism of isobutyrate by colonocytes. In: Archives of Biochemistry and Biophysics. 1996 ; Vol. 327, No. 2. pp. 265-270.
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