Catabolite repression of the colonization factor antigen I (CFA/I) operon of Escherichia coli

Tuomo K. Karjalainen, Dolores G. Evans, Doyle J. Evans, David Y. Graham, Chao-Hung Lee

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Experiments were performed to study whether the synthesis of the fimbrial colonization factor antigen I (CFA/I) of enterotoxigenic Escherichia coli is affected by glucose. The CFA/I-producing strain H-10407 (O78:H11:CFA/I) was grown in CFA medium containing various concentrations of glucose. Addition of 1% glucose into the medium resulted in a pronounced decrease in CFA/I production by H-10407 as assessed by ELISA, hemagglutination, and electron microscopy. The repressive effect of glucose was reversed by the addition of 10 m M cAMP to the medium. Examination of the promoter sequence of the cfaA gene of the CFA/I operon revealed a consensus binding site for the catabolite activator protein-cAMP complex. With a reporter plasmid containing a fusion of the cfaA promoter, a portion of the cfaA gene, and the lacZ gene, it was shown that the activity of this promoter was influenced by glucose. In a wild-type E. coli strain, addition of 0.5% glucose to the growth medium diminished the promoter activity more than 70%. The cfaA promoter also exhibited a lower level of activity in cya (adenyl cyclase) and crp (cAMP receptor protein) mutants than in the wild-type strain. The addition of 10 m M cAMP resulted in a marked increase in the expression from the cfaA promoter in the cya but not in the crp mutant. These results suggest that the suppressive effect of glucose in the CFA/I system is mediated via the mechanism of catabolite repression through the cfaA promoter of the CFA/I operon.

Original languageEnglish
Pages (from-to)307-313
Number of pages7
JournalCurrent Microbiology
Volume23
Issue number6
DOIs
StatePublished - Dec 1991

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Catabolite Repression
Operon
Escherichia coli
Glucose
Cyclic AMP Receptor Protein
Adenylyl Cyclases
Enterotoxigenic Escherichia coli
Lac Operon
Hemagglutination
colonization factor antigens
Genes
Electron Microscopy
Plasmids
Enzyme-Linked Immunosorbent Assay
Binding Sites
Growth

ASJC Scopus subject areas

  • Microbiology

Cite this

Karjalainen, T. K., Evans, D. G., Evans, D. J., Graham, D. Y., & Lee, C-H. (1991). Catabolite repression of the colonization factor antigen I (CFA/I) operon of Escherichia coli. Current Microbiology, 23(6), 307-313. https://doi.org/10.1007/BF02104131

Catabolite repression of the colonization factor antigen I (CFA/I) operon of Escherichia coli. / Karjalainen, Tuomo K.; Evans, Dolores G.; Evans, Doyle J.; Graham, David Y.; Lee, Chao-Hung.

In: Current Microbiology, Vol. 23, No. 6, 12.1991, p. 307-313.

Research output: Contribution to journalArticle

Karjalainen, TK, Evans, DG, Evans, DJ, Graham, DY & Lee, C-H 1991, 'Catabolite repression of the colonization factor antigen I (CFA/I) operon of Escherichia coli', Current Microbiology, vol. 23, no. 6, pp. 307-313. https://doi.org/10.1007/BF02104131
Karjalainen, Tuomo K. ; Evans, Dolores G. ; Evans, Doyle J. ; Graham, David Y. ; Lee, Chao-Hung. / Catabolite repression of the colonization factor antigen I (CFA/I) operon of Escherichia coli. In: Current Microbiology. 1991 ; Vol. 23, No. 6. pp. 307-313.
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