CCAAT/Enhancer-binding Protein α Alters Histone H3 Acetylation at Large Subnuclear Domains

Wan Hui Zhang, Roopali Srihari, Richard Day, Fred Schaufele

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Transcriptional regulation is commonly associated with local levels of histone acetylation, which controls chromatin structure at specific genes or within contiguous chromosomal domains. Less well understood are the higher order determinants of histone acetylation. The transcription factor, CCAAT/enhancer-binding protein α (C/EBPα), concentrates at one higher order structure, the peri-centromeric chromatin, and regulates differentiation in many cell types, including pituitary cells. We used quantitative fluorescence microscopy to show that immunostained acetylated histone H3 is relatively absent from peri-centromeric domains visible as large structures in mouse pituitary progenitor GHFT1-5 cells. GHFT1-5 cells do not contain C/EBPα. We observed that expression of C/EBPα in GHFT1-5 cells leads to an increased level of acetylated histone H3, but not acetylated histone H4, at the peri-centromeric domains. Only transcriptionally active forms of C/EBPα altered histone acetylation at the peri-centromeric domain. The altered state of histone acetylation at large intranuclear domains may complement, counteract, or supercede the more gene-local activities of other transcription factors to coordinate C/EBPα-induced cellular differentiation.

Original languageEnglish (US)
Pages (from-to)40373-40376
Number of pages4
JournalJournal of Biological Chemistry
Volume276
Issue number44
DOIs
StatePublished - Nov 2 2001
Externally publishedYes

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CCAAT-Enhancer-Binding Proteins
Acetylation
Histones
Chromatin
Transcription Factors
Genes
Fluorescence microscopy
Fluorescence Microscopy

ASJC Scopus subject areas

  • Biochemistry

Cite this

CCAAT/Enhancer-binding Protein α Alters Histone H3 Acetylation at Large Subnuclear Domains. / Zhang, Wan Hui; Srihari, Roopali; Day, Richard; Schaufele, Fred.

In: Journal of Biological Chemistry, Vol. 276, No. 44, 02.11.2001, p. 40373-40376.

Research output: Contribution to journalArticle

Zhang, Wan Hui ; Srihari, Roopali ; Day, Richard ; Schaufele, Fred. / CCAAT/Enhancer-binding Protein α Alters Histone H3 Acetylation at Large Subnuclear Domains. In: Journal of Biological Chemistry. 2001 ; Vol. 276, No. 44. pp. 40373-40376.
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