CCAAT/enhancer binding protein binds and activates the promoter of the rat class I alcohol dehydrogenase gene

James J. Potter, Esteban Mezey, Robert J. Christy, David Crabb, Peter M. Stein, Vincent W. Yang

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

CCAAT/enhancer binding protein (C/EBP), a DNA binding protein originally isolated from rat liver, activates the transcription of a number of liver-specific genes. We studied the effect of C/EBP on the transcription of the rat class I alcohol dehydrogenase gene. Purified C/EBP was shown to bind to a segment of the rat class I alcohol dehydrogenase gene promoter between position -10 and -22. Nuclear extracts isolated from rat liver contained a heat stable factor(s) that also bound to this sequence. Cotransfection experiments in HepG2 cells showed transactivation of alcohol dehydrogenase promoter-CAT fusion constructs by an expression vector containing wild-type C/EBP. Expression vector containing a mutated C/EBP that failed to bind DNA also failed to activate the promoter. These experiments demonstrate that C/EBP expression can activate the rat class I alcohol dehydrogenase gene promoter.

Original languageEnglish
Pages (from-to)246-251
Number of pages6
JournalArchives of Biochemistry and Biophysics
Volume285
Issue number2
DOIs
StatePublished - 1991

Fingerprint

CCAAT-Enhancer-Binding Proteins
Alcohol Dehydrogenase
Rats
Genes
Liver
Transcription
Hep G2 Cells
DNA-Binding Proteins
Transcriptional Activation
Fusion reactions
Hot Temperature
Experiments
DNA

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

CCAAT/enhancer binding protein binds and activates the promoter of the rat class I alcohol dehydrogenase gene. / Potter, James J.; Mezey, Esteban; Christy, Robert J.; Crabb, David; Stein, Peter M.; Yang, Vincent W.

In: Archives of Biochemistry and Biophysics, Vol. 285, No. 2, 1991, p. 246-251.

Research output: Contribution to journalArticle

Potter, James J. ; Mezey, Esteban ; Christy, Robert J. ; Crabb, David ; Stein, Peter M. ; Yang, Vincent W. / CCAAT/enhancer binding protein binds and activates the promoter of the rat class I alcohol dehydrogenase gene. In: Archives of Biochemistry and Biophysics. 1991 ; Vol. 285, No. 2. pp. 246-251.
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