CCAAT/enhancer binding protein binds and activates the promoter of the rat class I alcohol dehydrogenase gene

James J. Potter, Esteban Mezey, Robert J. Christy, David W. Crabb, Peter M. Stein, Vincent W. Yang

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Abstract

CCAAT/enhancer binding protein (C/EBP), a DNA binding protein originally isolated from rat liver, activates the transcription of a number of liver-specific genes. We studied the effect of C/EBP on the transcription of the rat class I alcohol dehydrogenase gene. Purified C/EBP was shown to bind to a segment of the rat class I alcohol dehydrogenase gene promoter between position -10 and -22. Nuclear extracts isolated from rat liver contained a heat stable factor(s) that also bound to this sequence. Cotransfection experiments in HepG2 cells showed transactivation of alcohol dehydrogenase promoter-CAT fusion constructs by an expression vector containing wild-type C/EBP. Expression vector containing a mutated C/EBP that failed to bind DNA also failed to activate the promoter. These experiments demonstrate that C/EBP expression can activate the rat class I alcohol dehydrogenase gene promoter.

Original languageEnglish (US)
Pages (from-to)246-251
Number of pages6
JournalArchives of Biochemistry and Biophysics
Volume285
Issue number2
DOIs
StatePublished - Mar 1991

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ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology

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