CCCP-Induced LC3 lipidation depends on Atg9 whereas FIP200/Atg13 and Beclin 1/Atg14 are dispensable

Daohong Chen, Xi Chen, Min Li, Hao Zhang, Wen Xing Ding, Xiao-Ming Yin

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Treatment of cells with carbonyl cyanide m-chlorophenylhydrazone (CCCP), a mitochondrial proton gradient uncoupler, can result in mitochondrial damage and autophagy activation, which in turn eliminates the injured mitochondria in a Parkin-dependent way. How CCCP mobilizes the autophagy machinery is not fully understood. By analyzing a key autophagy step, LC3 lipidation, we examined the roles of two kinase complexes typically involved in the initiation and nucleation phases of autophagy, namely the ULK kinase complex (UKC) and the Beclin 1/Atg14 complex. We found that CCCP-induced LC3 lipidation could be independent of Beclin 1 and Atg14. In addition, deletion or knockdown of the UKC component FIP200 or Atg13 only led to a partial reduction in LC3 lipidation, indicating that UKC could be also dispensable for this step during CCCP treatment. In contrast, Atg9, which is important for transporting vesicles to early autophagosomal structure, was required for CCCP-induced LC3 lipidation. Taken together, these data suggest that CCCP-induced autophagy and mitophagy depends more critically on Atg9 vesicles than on UKC and Beclin 1/Atg14 complex.

Original languageEnglish
Pages (from-to)226-230
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume432
Issue number2
DOIs
StatePublished - Mar 8 2013

Fingerprint

Autophagy
Phosphotransferases
Mitochondrial Degradation
Mitochondria
Machinery
Beclin-1
mesoxalonitrile
carbonyl 3-chlorophenylhydrazone
Protons
Nucleation
Chemical activation
Cells

Keywords

  • Atg13
  • Atg14
  • Atg9
  • Autophagy
  • Beclin 1
  • CCCP
  • FIP200
  • Mitophagy

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Molecular Biology

Cite this

CCCP-Induced LC3 lipidation depends on Atg9 whereas FIP200/Atg13 and Beclin 1/Atg14 are dispensable. / Chen, Daohong; Chen, Xi; Li, Min; Zhang, Hao; Ding, Wen Xing; Yin, Xiao-Ming.

In: Biochemical and Biophysical Research Communications, Vol. 432, No. 2, 08.03.2013, p. 226-230.

Research output: Contribution to journalArticle

Chen, Daohong ; Chen, Xi ; Li, Min ; Zhang, Hao ; Ding, Wen Xing ; Yin, Xiao-Ming. / CCCP-Induced LC3 lipidation depends on Atg9 whereas FIP200/Atg13 and Beclin 1/Atg14 are dispensable. In: Biochemical and Biophysical Research Communications. 2013 ; Vol. 432, No. 2. pp. 226-230.
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