CD1d expression on and regulation of murine hematopoietic stem and progenitor cells

Hal E. Broxmeyer, Kent Christopherson, Giao Hangoc, Scott Cooper, Charlie Mantel, Gourapura J. Renukaradhya, Randy R. Brutkiewicz

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

In the present study, surface CD1d, which is involved in immune cell interactions, was assessed for effects on hematopoiesis. Mouse BM hematopoietic stem cells (HSCs) and hematopoietic progenitor cells (HPCs) express CD1d. The numbers and cycling status of HPCs in the BM and spleen of different strains of cd1d-/- mice were enhanced significantly, suggesting that CD1d is a negative regulator of HPCs. In support of this, CD1d was required for the SCF and Flt3 ligand synergistic enhancement of CSF induction of HPC colony formation and for HPCresponse to myelosuppressive chemokines. Colony formation by immature subsets of HPCs was greatly enhanced when normal, but not cd1d-/-, BM cells were pretreated with CD1dAbs in vitro. These effects required the full CD1d cytoplasmic tail. In contrast, long-term, but not short-term, repopulating HSC engraftment was impaired significantly, an effect that was minimally influenced by the presence of a truncated CD1d cytoplasmic tail. Pretreatment of normal BM cells with CD1d Abs greatly enhanced their engraftment of HSCs. The results of the present study implicate CD1d in a previously unrecognized regulatory role of normal and stressed hematopoiesis.

Original languageEnglish (US)
Pages (from-to)5731-5741
Number of pages11
JournalBlood
Volume119
Issue number24
DOIs
StatePublished - Jun 14 2012

ASJC Scopus subject areas

  • Biochemistry
  • Immunology
  • Hematology
  • Cell Biology

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