CD27/CD70 interaction directly induces natural killer cell killing activity

F. C. Yang, K. Agematsu, T. Nakazawa, T. Mori, S. Ito, T. Kobata, C. Morimoto, A. Komiyama

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Abstract

The CD27 molecule is expressed on a portion of natural killer (NK) cells as well as T and B cells. To provide the functional capacity of CD27 molecule on NK cells, we here highly purified CD3- CD56+ NK cells by flow cytometry (purity >98%), and investigated their NK cell activity via CD27/CD70 interaction using a CD70-transfectant by a 4 h 51Cr-release assay. The enhancement of NK activity by purified NK cells in the presence of interleukin-2 (IL-2) or interleukin-12 (IL-12) against CD70/Nalm-6 was not recognized as compared to against mock/Nalm-6. However, after a coculture with the fixed CD70/300 19, the purified NK cells increased the NK cell activity against K562, the value being 10 to 20% higher than coculture with the mock/300-19 in the presence of IL-2 or IL-12. The enhancement of NK activity was blocked by the addition of anti-CD70 monoclonal antibody (mAb). In addition, conjugation of NK cells to the target was increased by coculture with the CD70/300 19 without increased expression of adhesion molecules. In the parallel experiment, there was no increase in the killing capacity of NK cells. These results strongly show that CD27/CD70 interaction directly enhances NK activity in the presence of IL-2 or IL-12 by increasing the effector and target conjugate formation, indicating that CD27/CD70 interaction plays an important role in the cytotoxic function of NK cells.

Original languageEnglish (US)
Pages (from-to)289-293
Number of pages5
JournalImmunology
Volume88
Issue number2
StatePublished - 1996
Externally publishedYes

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Natural Killer Cells
Interleukin-12
Coculture Techniques
Interleukin-2
Flow Cytometry
B-Lymphocytes
Monoclonal Antibodies
T-Lymphocytes

ASJC Scopus subject areas

  • Immunology

Cite this

Yang, F. C., Agematsu, K., Nakazawa, T., Mori, T., Ito, S., Kobata, T., ... Komiyama, A. (1996). CD27/CD70 interaction directly induces natural killer cell killing activity. Immunology, 88(2), 289-293.

CD27/CD70 interaction directly induces natural killer cell killing activity. / Yang, F. C.; Agematsu, K.; Nakazawa, T.; Mori, T.; Ito, S.; Kobata, T.; Morimoto, C.; Komiyama, A.

In: Immunology, Vol. 88, No. 2, 1996, p. 289-293.

Research output: Contribution to journalArticle

Yang, FC, Agematsu, K, Nakazawa, T, Mori, T, Ito, S, Kobata, T, Morimoto, C & Komiyama, A 1996, 'CD27/CD70 interaction directly induces natural killer cell killing activity', Immunology, vol. 88, no. 2, pp. 289-293.
Yang FC, Agematsu K, Nakazawa T, Mori T, Ito S, Kobata T et al. CD27/CD70 interaction directly induces natural killer cell killing activity. Immunology. 1996;88(2):289-293.
Yang, F. C. ; Agematsu, K. ; Nakazawa, T. ; Mori, T. ; Ito, S. ; Kobata, T. ; Morimoto, C. ; Komiyama, A. / CD27/CD70 interaction directly induces natural killer cell killing activity. In: Immunology. 1996 ; Vol. 88, No. 2. pp. 289-293.
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