CD34+++ stem/progenitor cells purified from cryopreserved normal cord blood can be transduced with high efficiency by a retroviral vector and expanded ex vivo with stable integration and expression of fanconi anemia complementation C gene

Li Lu, Yue Ge, Zhi Hua Li, Brian Freie, D. Wade Clapp, Hal E. Broxmeyer

Research output: Contribution to journalArticle

22 Scopus citations


A future possibility for treatment of genetic diseases may be gene therapy using autologous cord blood (CB) stem/progenitor cells. This might require cryopreservation of CB stem/progenitor cells prior to purification, gene transduction, and ex vivo expansion of cells. To address this possibility, nonadherent low density T-lymphocyte depleted (NALT-) cells from fresh or cryopreserved cord blood were sorted for CD34+++ phenotype, transduced with a recombinant retroviral vector encoding Fanconi anemia complementation C (FACC) gene, and cells expanded ex vivo in suspension culture for 7 days with growth factors. The results demonstrate: 1) high recovery of viable cells after thawing; 2) high efficiency purification of CD34+++ cells from NALT- cells prior to and after cryopreservation; 3) high degree of expansion of nucleated cells and immature progenitors from CD34+++ cells before and after cryopreservation; 4) efficient transduction with stable integration and expression of newly introduced genes in cryopreserved and then sorted stem/progenitor cells, as detected prior to and after ex vivo expansion; and 5) high efficiency transduction of single isolated CD34+++ cells obtained from cryopreserved NALT- CB. This information should be of value for future studies evaluating the use of cryopreserved cord blood for gene transfer/gene therapy.

Original languageEnglish (US)
Pages (from-to)493-503
Number of pages11
JournalCell Transplantation
Issue number5
StatePublished - Jan 1 1995



  • Cord blood
  • Cryopreservation
  • Gene transduction
  • Gene transfer
  • Hematopoietic stem/progenitors
  • Retrovirus

ASJC Scopus subject areas

  • Cell Biology
  • Biomedical Engineering
  • Transplantation

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