CD4+ Epstein-Barr virus-specific cytotoxic T-lymphocytes from human umbilical cord blood

Qi Sun, Robert L. Burton, Karen Pollok, David J. Emanuel, Kenneth G. Lucas

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

Umbilical cord blood (CB) is increasingly used for allogeneic hematopoietic stem cell transplantation. To determine whether viral antigen- specific cytotoxic T-lymphocytes (CTL) could be generated from the predominantly naive T-cell populations in CB, CB-derived mononuclear cells were stimulated with autologous Epstein-Barr virus (EBV) transformed B- lymphoblastoid cell lines over several weeks in the presence of recombinant human interleukin-2 (IL-2). By 28 days of culture, T-lymphocytes from all six CB that had been treated with IL-2 displayed EBV-specific cytotoxicity. These cells were largely CD4+, with complete inhibition of cytotoxicity by anti- CD3 and variable inhibition by anti-HLA DR monoclonal antibodies. The EBV- specific effectors were cloned by limiting dilution, and most of the CTL clones were CD4+. The cytotoxicity of the CB-derived CD4+ CTL clones was inhibited by EGTA but not by anti-Fas ligand mAb, suggesting that this cytotoxicity was mediated by perforin/granzyme B. These data indicate that virus-specific CTL can be cultivated and cloned from CB, a human T-cell source that may not have prior in vivo antigenic exposure or reactivity. This finding may have applications in adoptive immunotherapy to recipients of CB transplants.

Original languageEnglish
Pages (from-to)81-88
Number of pages8
JournalCellular Immunology
Volume195
Issue number2
DOIs
StatePublished - Aug 1 1999

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Cytotoxic T-Lymphocytes
Human Herpesvirus 4
Fetal Blood
T-Lymphocytes
Interleukin-2
Clone Cells
Adoptive Immunotherapy
Fas Ligand Protein
Viral Antigens
Egtazic Acid
Hematopoietic Stem Cell Transplantation
HLA-DR Antigens
Monoclonal Antibodies
Viruses
Transplants
Cell Line
Population

ASJC Scopus subject areas

  • Cell Biology
  • Immunology

Cite this

CD4+ Epstein-Barr virus-specific cytotoxic T-lymphocytes from human umbilical cord blood. / Sun, Qi; Burton, Robert L.; Pollok, Karen; Emanuel, David J.; Lucas, Kenneth G.

In: Cellular Immunology, Vol. 195, No. 2, 01.08.1999, p. 81-88.

Research output: Contribution to journalArticle

Sun, Qi ; Burton, Robert L. ; Pollok, Karen ; Emanuel, David J. ; Lucas, Kenneth G. / CD4+ Epstein-Barr virus-specific cytotoxic T-lymphocytes from human umbilical cord blood. In: Cellular Immunology. 1999 ; Vol. 195, No. 2. pp. 81-88.
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