CD4+ T, but not CD8+ or B, lymphocytes mediate facial motoneuron survival after facial nerve transection

Craig J. Serpe, Susanna Coers, Virginia M. Sanders, Kathryn J. Jones

Research output: Contribution to journalArticle

87 Citations (Scopus)

Abstract

The capacity of facial motor neurons (FMN) to survive injury and successfully regenerate is substantially compromised in immunodeficient mice, which lack T and B lymphocytes (Serpe et al., 1999). The goal of the present study was to determine which T cell subset (CD4+ and/or CD8+), and whether the B lymphocyte, is involved in FMN survival after nerve injury. All mice were subjected to a right facial nerve axotomy, with the left (uncut) side serving as an internal control. FMN survival, of the right (cut) side, was measured 4 weeks post-operative, and expressed as a percentage of the left (uncut) control side. FMN survival in wild-type mice was 86%±1.5. In contrast, FMN survival in CD4 KO mice was 60%±2.0. Reconstitution of either CD4 KO mice, or recombinase activating gene-2 knockout (RAG-2 KO) mice (which lack functional T and B cells) with CD4+ T cells alone restored FMN survival to wild-type levels (85%±1.2 and 84%±2.5, respectively). There was no difference in FMN survival between wild-type, CD8 KO and MμMT (B cell deficient) mice. Reconstitution of RAG-2 KO mice with CD8+ T cells alone, or B cells alone, failed to restore FMN survival levels (65%±1.5 and 63%±1.0, respectively). It is concluded that, of the population of FMN that do not survive injury, CD4+ T lymphocytes, but not CD8+ T lymphocytes or B cells, mediate FMN survival after peripheral nerve injury.

Original languageEnglish (US)
Pages (from-to)393-402
Number of pages10
JournalBrain, Behavior, and Immunity
Volume17
Issue number5
DOIs
StatePublished - Oct 2003

Fingerprint

Facial Nerve
Motor Neurons
B-Lymphocytes
T-Lymphocytes
Gene Knockout Techniques
Recombinases
Knockout Mice
Wounds and Injuries
Axotomy
Peripheral Nerve Injuries
T-Lymphocyte Subsets

Keywords

  • Acquired immunity
  • FMN
  • Neuro-immune interactions
  • Neuronal survival
  • Reconstitution
  • T and B Lymphocytes

ASJC Scopus subject areas

  • Immunology
  • Endocrine and Autonomic Systems
  • Behavioral Neuroscience

Cite this

CD4+ T, but not CD8+ or B, lymphocytes mediate facial motoneuron survival after facial nerve transection. / Serpe, Craig J.; Coers, Susanna; Sanders, Virginia M.; Jones, Kathryn J.

In: Brain, Behavior, and Immunity, Vol. 17, No. 5, 10.2003, p. 393-402.

Research output: Contribution to journalArticle

@article{f275e45abdba4ec298c7c21bb795043e,
title = "CD4+ T, but not CD8+ or B, lymphocytes mediate facial motoneuron survival after facial nerve transection",
abstract = "The capacity of facial motor neurons (FMN) to survive injury and successfully regenerate is substantially compromised in immunodeficient mice, which lack T and B lymphocytes (Serpe et al., 1999). The goal of the present study was to determine which T cell subset (CD4+ and/or CD8+), and whether the B lymphocyte, is involved in FMN survival after nerve injury. All mice were subjected to a right facial nerve axotomy, with the left (uncut) side serving as an internal control. FMN survival, of the right (cut) side, was measured 4 weeks post-operative, and expressed as a percentage of the left (uncut) control side. FMN survival in wild-type mice was 86{\%}±1.5. In contrast, FMN survival in CD4 KO mice was 60{\%}±2.0. Reconstitution of either CD4 KO mice, or recombinase activating gene-2 knockout (RAG-2 KO) mice (which lack functional T and B cells) with CD4+ T cells alone restored FMN survival to wild-type levels (85{\%}±1.2 and 84{\%}±2.5, respectively). There was no difference in FMN survival between wild-type, CD8 KO and MμMT (B cell deficient) mice. Reconstitution of RAG-2 KO mice with CD8+ T cells alone, or B cells alone, failed to restore FMN survival levels (65{\%}±1.5 and 63{\%}±1.0, respectively). It is concluded that, of the population of FMN that do not survive injury, CD4+ T lymphocytes, but not CD8+ T lymphocytes or B cells, mediate FMN survival after peripheral nerve injury.",
keywords = "Acquired immunity, FMN, Neuro-immune interactions, Neuronal survival, Reconstitution, T and B Lymphocytes",
author = "Serpe, {Craig J.} and Susanna Coers and Sanders, {Virginia M.} and Jones, {Kathryn J.}",
year = "2003",
month = "10",
doi = "10.1016/S0889-1591(03)00028-X",
language = "English (US)",
volume = "17",
pages = "393--402",
journal = "Brain, Behavior, and Immunity",
issn = "0889-1591",
publisher = "Academic Press Inc.",
number = "5",

}

TY - JOUR

T1 - CD4+ T, but not CD8+ or B, lymphocytes mediate facial motoneuron survival after facial nerve transection

AU - Serpe, Craig J.

AU - Coers, Susanna

AU - Sanders, Virginia M.

AU - Jones, Kathryn J.

PY - 2003/10

Y1 - 2003/10

N2 - The capacity of facial motor neurons (FMN) to survive injury and successfully regenerate is substantially compromised in immunodeficient mice, which lack T and B lymphocytes (Serpe et al., 1999). The goal of the present study was to determine which T cell subset (CD4+ and/or CD8+), and whether the B lymphocyte, is involved in FMN survival after nerve injury. All mice were subjected to a right facial nerve axotomy, with the left (uncut) side serving as an internal control. FMN survival, of the right (cut) side, was measured 4 weeks post-operative, and expressed as a percentage of the left (uncut) control side. FMN survival in wild-type mice was 86%±1.5. In contrast, FMN survival in CD4 KO mice was 60%±2.0. Reconstitution of either CD4 KO mice, or recombinase activating gene-2 knockout (RAG-2 KO) mice (which lack functional T and B cells) with CD4+ T cells alone restored FMN survival to wild-type levels (85%±1.2 and 84%±2.5, respectively). There was no difference in FMN survival between wild-type, CD8 KO and MμMT (B cell deficient) mice. Reconstitution of RAG-2 KO mice with CD8+ T cells alone, or B cells alone, failed to restore FMN survival levels (65%±1.5 and 63%±1.0, respectively). It is concluded that, of the population of FMN that do not survive injury, CD4+ T lymphocytes, but not CD8+ T lymphocytes or B cells, mediate FMN survival after peripheral nerve injury.

AB - The capacity of facial motor neurons (FMN) to survive injury and successfully regenerate is substantially compromised in immunodeficient mice, which lack T and B lymphocytes (Serpe et al., 1999). The goal of the present study was to determine which T cell subset (CD4+ and/or CD8+), and whether the B lymphocyte, is involved in FMN survival after nerve injury. All mice were subjected to a right facial nerve axotomy, with the left (uncut) side serving as an internal control. FMN survival, of the right (cut) side, was measured 4 weeks post-operative, and expressed as a percentage of the left (uncut) control side. FMN survival in wild-type mice was 86%±1.5. In contrast, FMN survival in CD4 KO mice was 60%±2.0. Reconstitution of either CD4 KO mice, or recombinase activating gene-2 knockout (RAG-2 KO) mice (which lack functional T and B cells) with CD4+ T cells alone restored FMN survival to wild-type levels (85%±1.2 and 84%±2.5, respectively). There was no difference in FMN survival between wild-type, CD8 KO and MμMT (B cell deficient) mice. Reconstitution of RAG-2 KO mice with CD8+ T cells alone, or B cells alone, failed to restore FMN survival levels (65%±1.5 and 63%±1.0, respectively). It is concluded that, of the population of FMN that do not survive injury, CD4+ T lymphocytes, but not CD8+ T lymphocytes or B cells, mediate FMN survival after peripheral nerve injury.

KW - Acquired immunity

KW - FMN

KW - Neuro-immune interactions

KW - Neuronal survival

KW - Reconstitution

KW - T and B Lymphocytes

UR - http://www.scopus.com/inward/record.url?scp=0041942742&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0041942742&partnerID=8YFLogxK

U2 - 10.1016/S0889-1591(03)00028-X

DO - 10.1016/S0889-1591(03)00028-X

M3 - Article

C2 - 12946661

AN - SCOPUS:0041942742

VL - 17

SP - 393

EP - 402

JO - Brain, Behavior, and Immunity

JF - Brain, Behavior, and Immunity

SN - 0889-1591

IS - 5

ER -