CD40 promotes mhc class II expression on adipose tissue macrophages and regulates adipose tissue CD4+ T cells with obesity

David L. Morris, Kelsie E. Oatmen, Taleen A. Mergian, Kae Won Cho, Jennifer L. Delproposto, Kanakadurga Singer, Carmella Evans-Molina, Robert W. O’Rourke, Carey N. Lumeng

Research output: Contribution to journalArticle

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Abstract

Obesity activates both innate and adaptive immune responses in adipose tissue, but the mechanisms critical for regulating these responses remain unknown. CD40/CD40L signaling provides bidirectional costimulatory signals between antigen-presenting cells and CD4+ T cells, and CD40L expression is increased in obese humans. Therefore, we examined the contribution of CD40 to the progression of obesity-induced inflammation in mice. CD40 was highly expressed on adipose tissue macrophages in mice, and CD40/CD40L signaling promoted the expression of antigen-presenting cell markers in adipose tissue macrophages. When fed a high fat diet, Cd40-deficient mice had reduced accumulation of conventional CD4+ T cells (Tconv: CD3+CD4+Foxp3-) in visceral fat compared with wild-type mice. By contrast, the number of regulatory CD4+ T cells (Treg: CD3+CD4+ Foxp3+) in lean and obese fat was similar between wildtype and knockout mice. Adipose tissue macrophage content and inflammatory gene expression in fat did not differ between obese wild-type and knockout mice; however, major histocompatibility complex class II and CD86 expression on adipose tissue macrophages was reduced in visceral fat fromknockout mice. Similar results were observed in chimeric mice with hematopoietic Cd40-deficiency. Nonetheless, neither whole body nor hematopoietic disruption of CD40 ameliorated obesityinduced insulin resistance in mice. In human adipose tissue, CD40 expression was positively correlated with CD80 and CD86 expression in obese patients with type 2 diabetes. These findings indicate that CD40 signaling in adipose tissue macrophages regulates major histocompatibility complex class II and CD86 expression to control the expansion of CD4+ T cells; however, this is largely dispensable for the development of obesity-induced inflammation and insulin resistance in mice.

Original languageEnglish (US)
Pages (from-to)1107-1119
Number of pages13
JournalJournal of Leukocyte Biology
Volume99
Issue number6
DOIs
StatePublished - Jun 1 2016

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Adipose Tissue
Obesity
Macrophages
T-Lymphocytes
CD40 Ligand
Intra-Abdominal Fat
Antigen-Presenting Cells
Major Histocompatibility Complex
Knockout Mice
Insulin Resistance
Fats
Inflammation
Obese Mice
High Fat Diet
Adaptive Immunity
Regulatory T-Lymphocytes
Innate Immunity
Type 2 Diabetes Mellitus
Gene Expression

Keywords

  • Antigen presenting cells (APC)
  • CD40L
  • Inflammation

ASJC Scopus subject areas

  • Cell Biology
  • Immunology

Cite this

Morris, D. L., Oatmen, K. E., Mergian, T. A., Cho, K. W., Delproposto, J. L., Singer, K., ... Lumeng, C. N. (2016). CD40 promotes mhc class II expression on adipose tissue macrophages and regulates adipose tissue CD4+ T cells with obesity. Journal of Leukocyte Biology, 99(6), 1107-1119. https://doi.org/10.1189/jlb.3A0115-009R

CD40 promotes mhc class II expression on adipose tissue macrophages and regulates adipose tissue CD4+ T cells with obesity. / Morris, David L.; Oatmen, Kelsie E.; Mergian, Taleen A.; Cho, Kae Won; Delproposto, Jennifer L.; Singer, Kanakadurga; Evans-Molina, Carmella; O’Rourke, Robert W.; Lumeng, Carey N.

In: Journal of Leukocyte Biology, Vol. 99, No. 6, 01.06.2016, p. 1107-1119.

Research output: Contribution to journalArticle

Morris, DL, Oatmen, KE, Mergian, TA, Cho, KW, Delproposto, JL, Singer, K, Evans-Molina, C, O’Rourke, RW & Lumeng, CN 2016, 'CD40 promotes mhc class II expression on adipose tissue macrophages and regulates adipose tissue CD4+ T cells with obesity', Journal of Leukocyte Biology, vol. 99, no. 6, pp. 1107-1119. https://doi.org/10.1189/jlb.3A0115-009R
Morris, David L. ; Oatmen, Kelsie E. ; Mergian, Taleen A. ; Cho, Kae Won ; Delproposto, Jennifer L. ; Singer, Kanakadurga ; Evans-Molina, Carmella ; O’Rourke, Robert W. ; Lumeng, Carey N. / CD40 promotes mhc class II expression on adipose tissue macrophages and regulates adipose tissue CD4+ T cells with obesity. In: Journal of Leukocyte Biology. 2016 ; Vol. 99, No. 6. pp. 1107-1119.
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