CD41 expression defines the onset of primitive and definitive hematopoiesis in the murine embryo

Michael J. Ferkowicz, Mark Starr, Xiaodong Xie, Weiming Li, Scott A. Johnson, William C. Shelley, Paul R. Morrison, Mervin Yoder

Research output: Contribution to journalArticle

235 Citations (Scopus)

Abstract

The platelet glycoprotein IIb (αIIb; CD41) constitutes the alpha subunit of a highly expressed platelet surface integrin protein. We demonstrate that CD41 serves as the earliest marker of primitive erythroid progenitor cells in the embryonic day 7 (E7.0) yolk sac and high-level expression identifies essentially all E8.25 yolk sac definitive hematopoietic progenitors. Some definitive hematopoietic progenitor cells in the fetal liver and bone marrow also express CD41. Hematopoietic stem cell competitive repopulating ability is present in CD41dim and CD41lo/- cells isolated from bone marrow and fetal liver cells, however, activity is enriched in the CD41lo/- cells. CD41bright yolk sac definitive progenitor cells co-express CD61 and bind fibrinogen, demonstrating receptor function. Thus, CD41 expression marks the onset of primitive and definitive hematopoiesis in the murine embryo and persists as a marker of some stem and progenitor cell populations in the fetal liver and adult marrow, suggesting novel roles for this integrin.

Original languageEnglish
Pages (from-to)4393-4403
Number of pages11
JournalDevelopment (Cambridge)
Volume130
Issue number18
DOIs
StatePublished - Sep 2003

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Yolk Sac
Hematopoiesis
Stem Cells
Embryonic Structures
Hematopoietic Stem Cells
Integrins
Liver
Bone Marrow
Fibrinogen Receptors
Platelet Membrane Glycoproteins
Erythroid Precursor Cells
Bone Marrow Cells
Membrane Proteins
Blood Platelets
Population

Keywords

  • CD41
  • Embryonic yolk sac
  • Hematopoiesis
  • Mouse

ASJC Scopus subject areas

  • Anatomy
  • Cell Biology

Cite this

CD41 expression defines the onset of primitive and definitive hematopoiesis in the murine embryo. / Ferkowicz, Michael J.; Starr, Mark; Xie, Xiaodong; Li, Weiming; Johnson, Scott A.; Shelley, William C.; Morrison, Paul R.; Yoder, Mervin.

In: Development (Cambridge), Vol. 130, No. 18, 09.2003, p. 4393-4403.

Research output: Contribution to journalArticle

Ferkowicz, MJ, Starr, M, Xie, X, Li, W, Johnson, SA, Shelley, WC, Morrison, PR & Yoder, M 2003, 'CD41 expression defines the onset of primitive and definitive hematopoiesis in the murine embryo', Development (Cambridge), vol. 130, no. 18, pp. 4393-4403. https://doi.org/10.1242/dev.00632
Ferkowicz, Michael J. ; Starr, Mark ; Xie, Xiaodong ; Li, Weiming ; Johnson, Scott A. ; Shelley, William C. ; Morrison, Paul R. ; Yoder, Mervin. / CD41 expression defines the onset of primitive and definitive hematopoiesis in the murine embryo. In: Development (Cambridge). 2003 ; Vol. 130, No. 18. pp. 4393-4403.
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