CD44 differentially activates mouse NK T cells and conventional T cells

Jonathan Larkin, Gourapura J. Renukaradhya, Venkataraman Sriram, Wenjun Du, Jacquelyn Gervay-Hague, Randy Brutkiewicz

Research output: Contribution to journalArticle

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Abstract

NK T (NKT) cells are an important component of the innate immune system and recognize the MHC class I-like CD1d molecule. NKT cells possess significant immunoregulatory activity due to their rapid secretion of large quantities of pro- and antiinflammatory cytokines following Cd1d-dependent stimulation. Because the innate immune system is programmed to respond to a multitude of diverse stimuli and must be able to quickly differentiate between pathogenic and endogenous signals, we hypothesized that, apart from stimulation via the TCR (e.g., CD1d-dependent activation), there must be multiple activation pathways that can be triggered through other cell surface receptors on NKT cells. Therefore, we analyzed the ability of CD44, a structurally diverse cell surface receptor expressed on most cells, to stimulate murine NKT cells, compared with conventional T cells. Stimulation of CD44 through Ab cross-linking or binding to its natural ligands hyaluronan and osteopontin induced NKT cells to secrete cytokines, up-regulate activation markers, undergo morphological changes, and resist activation-induced cell death, whereas conventional T cells only exhibited changes in morphology and protection from activation-induced cell death. This CD44-specific stimulation of NKT cells correlated with their ability to bind hyaluronan. Thus, fundamental differences in CD44 function between these lymphocyte subsets suggest an important biological role for CD44 in the innate immune response.

Original languageEnglish
Pages (from-to)268-279
Number of pages12
JournalJournal of Immunology
Volume177
Issue number1
StatePublished - Jul 1 2006

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Natural Killer T-Cells
Natural Killer Cells
T-Lymphocytes
Cell Surface Receptors
Hyaluronic Acid
CD1d Antigen
Immune System
Cell Death
Cytokines
Osteopontin
Lymphocyte Subsets
Cellular Structures
Innate Immunity
Anti-Inflammatory Agents
Up-Regulation
Ligands

ASJC Scopus subject areas

  • Immunology

Cite this

Larkin, J., Renukaradhya, G. J., Sriram, V., Du, W., Gervay-Hague, J., & Brutkiewicz, R. (2006). CD44 differentially activates mouse NK T cells and conventional T cells. Journal of Immunology, 177(1), 268-279.

CD44 differentially activates mouse NK T cells and conventional T cells. / Larkin, Jonathan; Renukaradhya, Gourapura J.; Sriram, Venkataraman; Du, Wenjun; Gervay-Hague, Jacquelyn; Brutkiewicz, Randy.

In: Journal of Immunology, Vol. 177, No. 1, 01.07.2006, p. 268-279.

Research output: Contribution to journalArticle

Larkin, J, Renukaradhya, GJ, Sriram, V, Du, W, Gervay-Hague, J & Brutkiewicz, R 2006, 'CD44 differentially activates mouse NK T cells and conventional T cells', Journal of Immunology, vol. 177, no. 1, pp. 268-279.
Larkin J, Renukaradhya GJ, Sriram V, Du W, Gervay-Hague J, Brutkiewicz R. CD44 differentially activates mouse NK T cells and conventional T cells. Journal of Immunology. 2006 Jul 1;177(1):268-279.
Larkin, Jonathan ; Renukaradhya, Gourapura J. ; Sriram, Venkataraman ; Du, Wenjun ; Gervay-Hague, Jacquelyn ; Brutkiewicz, Randy. / CD44 differentially activates mouse NK T cells and conventional T cells. In: Journal of Immunology. 2006 ; Vol. 177, No. 1. pp. 268-279.
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