CD44+/CD24-Breast cancer cells exhibit enhanced invase properties

An early step necessary for metastasis

Carol Sheridan, Hiromitsu Kishimoto, Robyn K. Fuchs, Sanjana Mehrotra, Poornima Bhat-Nakshatri, Charles H. Turner, Robert Goulet, Sunil Badve, Harikrishna Nakshatri

Research output: Contribution to journalArticle

637 Citations (Scopus)

Abstract

Introduction: A subpopulation (CD44+/CD24-) of breast cancer cells has been reported to have stem/progenitor cell properties. The aim of this study was to investigate whether this subpopulation of cancer cells has the unique ability to invade, home, and proliferate at sites of metastasis. Methods: CD44 and CD24 expression was determined by flow cytometry. Northern blotting was used to determine the expression of proinvasive and 'bone and lung metastasis signature' genes. A matrigel invasion assay and intracardiac inoculation into nude mice were used to evaluate invasion, and homing and proliferation at sites of metastasis, respectively. Results: Five among 13 breast cancer cell lines examined (MDA-MB-231, MDA-MB-436, Hs578T, SUM1315, and HBL-100) contained a higher percentage (>30%) of CD44+/CD24- cells. Cell lines with high CD44+/CD24- cell numbers express basal/ mesenchymal or myoepithelial but not luminal markers. Expression levels of proinvasive genes (IL-1α, IL-6, IL-8, and urokinase plasminogen activator [UPA]) were higher in cell lines with a significant CD44+/CD24- population than in other cell lines. Among the CD44+/CD24--positive cell lines, MDA-MB-231 has the unique property of expressing a broad range of genes that favor bone and lung metastasis. Consistent with previous studies in nude mice, cell lines with CD44+/ CD24- subpopulation were more invasive than other cell lines. However, only a subset of CD44+/CD24--positive cell lines was able to home and proliferate in lungs. Conclusion: Breast cancer cells with CD44+/CD24- subpopulation express higher levels of proinvasive genes and have highly invasive properties. However, this phenotype is not sufficient to predict capacity for pulmonary metastasis.

Original languageEnglish
Article numberR59
JournalBreast Cancer Research
Volume8
Issue number5
DOIs
StatePublished - Oct 24 2006

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Breast Neoplasms
Neoplasm Metastasis
Cell Line
Lung
Nude Mice
Genes
Stem Cells
Bone and Bones
Plasminogen Activators
Urokinase-Type Plasminogen Activator
Interleukin-8
Interleukin-1
Northern Blotting
Interleukin-6
Flow Cytometry
Cell Count
Phenotype
Population
Neoplasms

ASJC Scopus subject areas

  • Cancer Research
  • Oncology
  • Medicine(all)

Cite this

CD44+/CD24-Breast cancer cells exhibit enhanced invase properties : An early step necessary for metastasis. / Sheridan, Carol; Kishimoto, Hiromitsu; Fuchs, Robyn K.; Mehrotra, Sanjana; Bhat-Nakshatri, Poornima; Turner, Charles H.; Goulet, Robert; Badve, Sunil; Nakshatri, Harikrishna.

In: Breast Cancer Research, Vol. 8, No. 5, R59, 24.10.2006.

Research output: Contribution to journalArticle

Sheridan, C, Kishimoto, H, Fuchs, RK, Mehrotra, S, Bhat-Nakshatri, P, Turner, CH, Goulet, R, Badve, S & Nakshatri, H 2006, 'CD44+/CD24-Breast cancer cells exhibit enhanced invase properties: An early step necessary for metastasis', Breast Cancer Research, vol. 8, no. 5, R59. https://doi.org/10.1186/bcr1610
Sheridan C, Kishimoto H, Fuchs RK, Mehrotra S, Bhat-Nakshatri P, Turner CH et al. CD44+/CD24-Breast cancer cells exhibit enhanced invase properties: An early step necessary for metastasis. Breast Cancer Research. 2006 Oct 24;8(5). R59. https://doi.org/10.1186/bcr1610
Sheridan, Carol ; Kishimoto, Hiromitsu ; Fuchs, Robyn K. ; Mehrotra, Sanjana ; Bhat-Nakshatri, Poornima ; Turner, Charles H. ; Goulet, Robert ; Badve, Sunil ; Nakshatri, Harikrishna. / CD44+/CD24-Breast cancer cells exhibit enhanced invase properties : An early step necessary for metastasis. In: Breast Cancer Research. 2006 ; Vol. 8, No. 5.
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abstract = "Introduction: A subpopulation (CD44+/CD24-) of breast cancer cells has been reported to have stem/progenitor cell properties. The aim of this study was to investigate whether this subpopulation of cancer cells has the unique ability to invade, home, and proliferate at sites of metastasis. Methods: CD44 and CD24 expression was determined by flow cytometry. Northern blotting was used to determine the expression of proinvasive and 'bone and lung metastasis signature' genes. A matrigel invasion assay and intracardiac inoculation into nude mice were used to evaluate invasion, and homing and proliferation at sites of metastasis, respectively. Results: Five among 13 breast cancer cell lines examined (MDA-MB-231, MDA-MB-436, Hs578T, SUM1315, and HBL-100) contained a higher percentage (>30{\%}) of CD44+/CD24- cells. Cell lines with high CD44+/CD24- cell numbers express basal/ mesenchymal or myoepithelial but not luminal markers. Expression levels of proinvasive genes (IL-1α, IL-6, IL-8, and urokinase plasminogen activator [UPA]) were higher in cell lines with a significant CD44+/CD24- population than in other cell lines. Among the CD44+/CD24--positive cell lines, MDA-MB-231 has the unique property of expressing a broad range of genes that favor bone and lung metastasis. Consistent with previous studies in nude mice, cell lines with CD44+/ CD24- subpopulation were more invasive than other cell lines. However, only a subset of CD44+/CD24--positive cell lines was able to home and proliferate in lungs. Conclusion: Breast cancer cells with CD44+/CD24- subpopulation express higher levels of proinvasive genes and have highly invasive properties. However, this phenotype is not sufficient to predict capacity for pulmonary metastasis.",
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T2 - An early step necessary for metastasis

AU - Sheridan, Carol

AU - Kishimoto, Hiromitsu

AU - Fuchs, Robyn K.

AU - Mehrotra, Sanjana

AU - Bhat-Nakshatri, Poornima

AU - Turner, Charles H.

AU - Goulet, Robert

AU - Badve, Sunil

AU - Nakshatri, Harikrishna

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Y1 - 2006/10/24

N2 - Introduction: A subpopulation (CD44+/CD24-) of breast cancer cells has been reported to have stem/progenitor cell properties. The aim of this study was to investigate whether this subpopulation of cancer cells has the unique ability to invade, home, and proliferate at sites of metastasis. Methods: CD44 and CD24 expression was determined by flow cytometry. Northern blotting was used to determine the expression of proinvasive and 'bone and lung metastasis signature' genes. A matrigel invasion assay and intracardiac inoculation into nude mice were used to evaluate invasion, and homing and proliferation at sites of metastasis, respectively. Results: Five among 13 breast cancer cell lines examined (MDA-MB-231, MDA-MB-436, Hs578T, SUM1315, and HBL-100) contained a higher percentage (>30%) of CD44+/CD24- cells. Cell lines with high CD44+/CD24- cell numbers express basal/ mesenchymal or myoepithelial but not luminal markers. Expression levels of proinvasive genes (IL-1α, IL-6, IL-8, and urokinase plasminogen activator [UPA]) were higher in cell lines with a significant CD44+/CD24- population than in other cell lines. Among the CD44+/CD24--positive cell lines, MDA-MB-231 has the unique property of expressing a broad range of genes that favor bone and lung metastasis. Consistent with previous studies in nude mice, cell lines with CD44+/ CD24- subpopulation were more invasive than other cell lines. However, only a subset of CD44+/CD24--positive cell lines was able to home and proliferate in lungs. Conclusion: Breast cancer cells with CD44+/CD24- subpopulation express higher levels of proinvasive genes and have highly invasive properties. However, this phenotype is not sufficient to predict capacity for pulmonary metastasis.

AB - Introduction: A subpopulation (CD44+/CD24-) of breast cancer cells has been reported to have stem/progenitor cell properties. The aim of this study was to investigate whether this subpopulation of cancer cells has the unique ability to invade, home, and proliferate at sites of metastasis. Methods: CD44 and CD24 expression was determined by flow cytometry. Northern blotting was used to determine the expression of proinvasive and 'bone and lung metastasis signature' genes. A matrigel invasion assay and intracardiac inoculation into nude mice were used to evaluate invasion, and homing and proliferation at sites of metastasis, respectively. Results: Five among 13 breast cancer cell lines examined (MDA-MB-231, MDA-MB-436, Hs578T, SUM1315, and HBL-100) contained a higher percentage (>30%) of CD44+/CD24- cells. Cell lines with high CD44+/CD24- cell numbers express basal/ mesenchymal or myoepithelial but not luminal markers. Expression levels of proinvasive genes (IL-1α, IL-6, IL-8, and urokinase plasminogen activator [UPA]) were higher in cell lines with a significant CD44+/CD24- population than in other cell lines. Among the CD44+/CD24--positive cell lines, MDA-MB-231 has the unique property of expressing a broad range of genes that favor bone and lung metastasis. Consistent with previous studies in nude mice, cell lines with CD44+/ CD24- subpopulation were more invasive than other cell lines. However, only a subset of CD44+/CD24--positive cell lines was able to home and proliferate in lungs. Conclusion: Breast cancer cells with CD44+/CD24- subpopulation express higher levels of proinvasive genes and have highly invasive properties. However, this phenotype is not sufficient to predict capacity for pulmonary metastasis.

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