CD4+ T cell-mediated facial motoneuron survival after injury: Distribution pattern of cell death and rescue throughout the extent of the facial motor nucleus

Minh Y. Canh, Craig J. Serpe, Virginia Sanders, Kathryn Jones

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

We have previously demonstrated that CD4+ T cells transiently rescue facial motoneurons (FMN) from axotomy-induced death in immunodeficient mice. Three subpopulations of motoneurons have been observed within the facial motor nucleus following axotomy: one that always survives axotomy (50%), one that is amenable to rescue from axotomy-induced death through the addition of neurotrophic factors or CD4+ T cells (30-40%), and one that always dies after axotomy (10-15%). The objective of this study was to anatomically map the extent of axotomy-induced cell death and immune cell rescue in the facial nucleus to study the differential survival capabilities of each subpopulation. Wild-type (WT) mice, recombinase activating gene 2 knockout (RAG-2 KO) mice, and RAG-2 KO mice reconstituted with CD4+ T cells were subjected to right facial nerve axotomy. At 4 weeks post-axotomy, topographical mapping of axotomy-induced cell death throughout the rostro-caudal extent of the facial nucleus was accomplished in accordance with previously published maps of the subnuclear arrangement of the facial neurons. The results indicate that all 3 subpopulations of FMN can be found in each of the subnuclear groups throughout the entire rostro-caudal extent of the facial nucleus. These data are discussed in context of recent work in amyotrophic lateral sclerosis, a fatal motoneuron disease.

Original languageEnglish (US)
Pages (from-to)93-99
Number of pages7
JournalJournal of Neuroimmunology
Volume181
Issue number1-2
DOIs
StatePublished - Dec 2006
Externally publishedYes

Fingerprint

Axotomy
Motor Neurons
Cell Death
T-Lymphocytes
Wounds and Injuries
Gene Knockout Techniques
Recombinases
Knockout Mice
Facial Nucleus
Facial Nerve
Nerve Growth Factors
Amyotrophic Lateral Sclerosis
Neurons

Keywords

  • CD4 T cell
  • Motoneuron survival
  • RAG-2 KO

ASJC Scopus subject areas

  • Immunology
  • Clinical Neurology
  • Immunology and Allergy
  • Neurology

Cite this

CD4+ T cell-mediated facial motoneuron survival after injury : Distribution pattern of cell death and rescue throughout the extent of the facial motor nucleus. / Canh, Minh Y.; Serpe, Craig J.; Sanders, Virginia; Jones, Kathryn.

In: Journal of Neuroimmunology, Vol. 181, No. 1-2, 12.2006, p. 93-99.

Research output: Contribution to journalArticle

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