CD94 ligation induces apoptosis in a subset of IL-2-stimulated NK cells

Hiroaki Ida, Michael Robertson, Stephan Voss, Jerome Ritz, Paul Anderson

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

CD94 (Kp43) is a member of the human C-type lectin superfamily encoding type II membrane glycoproteins expressed on NK cells and a subset of T cells. Ligation of CD94 has been shown to either potentiate or inhibit NK cell proliferation and cytolytic effector function. Here we show that CD94 ligation triggers apoptosis in IL-2-primed NK cells. Evidence for CD94-induced apoptosis includes: 1) chromalin condensation as measured by increased fluorescence of Hoechst dye, 2) induction of DNA fragmentation, and 3) characteristic morphology by transmission electron microscopy. IL-2 priming (at least 12 h) is required for activation-induced NK cell death triggered by CD94. Activation-induced NK cell death triggered by CD94 ligation is extremely rapid (DNA fragmentation is first observed at 120 min). Unlike activation-induced T cell death, it is not inhibited by neutralizing Abs reactive with TNF-α or Fas ligand. Our results suggest that CD94 may play a role in the elimination of activated NK cells during the transition from the innate to the Ag-specific immune response.

Original languageEnglish (US)
Pages (from-to)2154-2160
Number of pages7
JournalJournal of Immunology
Volume159
Issue number5
StatePublished - 1997
Externally publishedYes

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Natural Killer Cells
Interleukin-2
Ligation
Apoptosis
Cell Death
DNA Fragmentation
C-Type Lectins
Fas Ligand Protein
Membrane Glycoproteins
T-Lymphocyte Subsets
Transmission Electron Microscopy
Fluorescence
Cell Proliferation
T-Lymphocytes

ASJC Scopus subject areas

  • Immunology

Cite this

Ida, H., Robertson, M., Voss, S., Ritz, J., & Anderson, P. (1997). CD94 ligation induces apoptosis in a subset of IL-2-stimulated NK cells. Journal of Immunology, 159(5), 2154-2160.

CD94 ligation induces apoptosis in a subset of IL-2-stimulated NK cells. / Ida, Hiroaki; Robertson, Michael; Voss, Stephan; Ritz, Jerome; Anderson, Paul.

In: Journal of Immunology, Vol. 159, No. 5, 1997, p. 2154-2160.

Research output: Contribution to journalArticle

Ida, H, Robertson, M, Voss, S, Ritz, J & Anderson, P 1997, 'CD94 ligation induces apoptosis in a subset of IL-2-stimulated NK cells', Journal of Immunology, vol. 159, no. 5, pp. 2154-2160.
Ida, Hiroaki ; Robertson, Michael ; Voss, Stephan ; Ritz, Jerome ; Anderson, Paul. / CD94 ligation induces apoptosis in a subset of IL-2-stimulated NK cells. In: Journal of Immunology. 1997 ; Vol. 159, No. 5. pp. 2154-2160.
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